INVIVO IDENTIFICATION OF THE TRANSFORMING GENE-PRODUCT OF SIMIAN SARCOMA-VIRUS
INVIVO IDENTIFICATION OF THE TRANSFORMING GENE-PRODUCT OF SIMIAN SARCOMA-VIRUS
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DOI：
10.1126/science.6293053
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
AARONSON, SA
中科院分区：
文献类型：
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作者：
ROBBINS, KC;DEVARE, SG;AARONSON, SA
猴肉瘤病毒（SSV）缺失突变体构建从一个分子克隆含有整个感染性前病毒。对这些突变体的转染分析将SSV转化基因定位于病毒基因组的一个小区域，包括其细胞衍生序列（v-sis）。根据预测的SSV转化基因的氨基酸序列合成的肽的抗血清检测到一种28，000-道尔顿的蛋白质，该蛋白质在SSV转化的[小鼠NIH-3 T3]细胞中特异性表达，并且其大小对应于从v-sis编码序列预测的大小。命名为p28 sis的v-sis基因产物不是磷蛋白，也不具有可检测的蛋白激酶活性。这些发现将p28 sis与其他一些逆转录病毒onc蛋白区分开来。
Simian sarcoma virus (SSV) deletion mutants were constructed from a molecular clone containing the entire infectious provirus. Transfection analysis of these mutants localized the SSV transforming gene to a small region of the viral genome encompassing its cell-derived sequence (v-sis). Antiserum to a peptide synthesized on the basis of the predicted amino acid sequence of the SSV transforming gene detected a 28,000-dalton protein that was specifically expressed in SSV transformed [mouse NIH-3T3] cells and that corresponded in size to that predicted from the v-sis coding sequence. The v-sis gene product designated p28sis was not a phosphoprotein, nor did it possess detectable protein kinase activity. These findings distinguish p28sis from a number of other retroviral onc proteins.