Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation.
Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation.
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组蛋白 mRNA 浓度在转录和 mRNA 降解水平上受到调节。
DOI：
10.1073/pnas.80.7.1849
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发表时间：
1983
影响因子：
11.1
通讯作者：
Marzluff,WF
中科院分区：
文献类型：
--
作者：
Sittman,DB;Graves,RA;Marzluff,WF
羟基脲或阿糖胞苷处理培养细胞后，组蛋白mRNA水平迅速下降。复制的组蛋白变体的组蛋白信使核糖核酸被迅速破坏，半衰期为10-15分钟。DNA合成抑制剂处理后，组蛋白替换变异体H3.3编码的mRNA水平没有变化。除了组蛋白基因的快速破坏外，组蛋白基因的转录速度也降低了1/5。淋巴瘤细胞(S49)被环AMP滞留在G1期，产生并含有显著水平的组蛋白mRNA。羟基脲降低了G1期细胞的转录速度和组蛋白mRNA水平。
The levels of histone mRNA are rapidly reduced after treatment of cultured cells with hydroxyurea or cytosine arabinonucleoside. The histone mRNA for the replicative histone variants is destroyed rapidly, with a half-life of 10-15 min. The levels of mRNA coding for the replacement histone variant H3.3 were unchanged after treatment with DNA synthesis inhibitors. In addition to the rapid destruction of histone mRNA, there was a reduction to 1/5th in the rate of transcription of the histone genes. Lymphoma cells (S49) arrested in G1 by cyclic AMP produce and contain significant levels of histone mRNA. Hydroxyurea reduces the rate of transcription and the levels of histone mRNA in the G1-arrested cells.