Some characteristics of a protein-coated ODS column and its use for the determination of drugs by the direct injection analysis of plasma samples
Some characteristics of a protein-coated ODS column and its use for the determination of drugs by the direct injection analysis of plasma samples
复制标题
蛋白质包被 ODS 柱的一些特性及其在血浆样品直接进样分析药物测定中的应用
DOI：
10.1007/bf02687789
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发表时间：
1984
期刊：
影响因子：
1.7
通讯作者：
H. Imai
中科院分区：
文献类型：
--
作者：
Hisanobu Yoshida;I. Morita;G. Tamai;T. Masujima;T. Tsuru;N. Takai;H. Imai
结果发现，涂有变性血浆蛋白（蛋白质包被的ODS）的小孔ODS柱（蛋白质包被的ODS）不再从水溶液中吸附血浆蛋白，而是保留了天然ODS对疏水性小分子的特性。元素分析和氮解吸 (BET) 分析表明，蛋白质包被的 ODS 含有约 25 mg Proteing−1dry 树脂，孔径或孔体积与天然 ODS 相似。包被的变性蛋白质似乎吸附在多孔树脂的外表面上，在通常的反相洗脱条件下没有被洗脱。这种蛋白质包被的 ODS 柱可用作分析柱或预柱，用于分析血浆中的加标药物。所有加标药物（如阿霉素、甲氨蝶呤）的回收率几乎是定量的（98-102%），具有良好的重现性（c.v.，小于 2%）。鉴于其准确性和简单性，本方法可用于测定血浆中的总疏水性药物，即游离+结合到血浆蛋白的疏水性药物。
It was found that an ODS column of small pore (120Å) which was coated with denatured plasma proteins (protein-coated ODS) no longer adsorbed plasma proteins from aqueous solution but retained the characteristics of native ODS for small hydrophobic molecules. Elemental analysis and nitrogen desorption (BET) analysis showed that the protein-coated ODS contained ca 25 mg proteing−1dry resin and that the pore diameter or pore volume was similar to that of native ODS. The coated denatured proteins, which seemed to be adsorbed on the external surface of the porous resin, were not eluted under usual reverse-phase elution conditions. Operating as either an analytical column or a pre-column, this protein-coated ODS column was used to analyse spiked-drugs in plasma. The recovery of all the spiked-drugs (such as doxorubicin, methotrexate) was almost quantitative (98–102%) with good reproducibilities (c.v., less than 2%). The present method was useful for the determination of total, that is, free + bound-to-plasma-proteins, hydrophobic drugs in plasma in view of its accuracy and simplicity.