Probe sialidase substrate specificity using chemoenzymatically synthesized sialosides containing C9-modified sialic acid
Probe sialidase substrate specificity using chemoenzymatically synthesized sialosides containing C9-modified sialic acid
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DOI：
10.1039/c2cc17393j
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Chen, Xi
中科院分区：
文献类型：
--
作者：
Khedri, Zahra;Muthana, Musleh M.;Chen, Xi
使用有效的一锅三酶系统从C6修饰的甘露糖衍生物合成含有C9修饰的唾液酸的α 2-3-和α 2-6-连接的唾液酸半乳糖苷的文库。这些唾液酸苷用于高通量唾液酸酶底物特异性测定，以阐明C9-OH在唾液酸酶识别中的重要性。
A library of alpha 2-3- and alpha 2-6-linked sialyl galactosides containing C9-modified sialic acids was synthesized from C6-modified mannose derivatives using an efficient one-pot three-enzyme system. These sialosides were used in a high-throughput sialidase substrate specificity assay to elucidate the importance of C9-OH in sialidase recognition.