Isolation and expansion of c-kit-positive cardiac progenitor cells by magnetic cell sorting.
Isolation and expansion of c-kit-positive cardiac progenitor cells by magnetic cell sorting.
复制标题
DOI：
10.1007/978-1-4939-1047-2_4
复制
发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Davis, Michael E
中科院分区：
文献类型：
--
作者：
French, Kristin M;Davis, Michael E
细胞治疗技术是组织再生的一种有前途的选择;特别是在心脏衰竭等移植受供体可用性限制的情况下。已经检查了多种细胞类型的心肌再生，包括间充质干细胞（和其他骨髓来源的细胞），诱导多能干细胞，胚胎干细胞，心球来源的细胞和心脏祖细胞（CPC）。CPC是多能性和克隆形成性的，可以从成熟组织中收获，并且具有自体移植的明显优势，并且在临床环境中没有肿瘤形成。在这里，我们专注于大鼠CPC的分离，扩增和心肌分化。还提供了用于从小鼠和人组织分离的方案的简要修改。
Cell therapy techniques are a promising option for tissue regeneration; especially in cases such as heart failure where transplantation is limited by donor availability. Multiple cell types have been examined for myocardial regeneration, including mesenchymal stem cells (and other bone marrow-derived cells), induced pluripotent stem cells, embryonic stem cells, cardiosphere-derived cells, and cardiac progenitor cells (CPCs). CPCs are multipotent and clonogenic, can be harvested from mature tissue, and have the distinct advantages of autologous transplant and lack of tumor formation in a clinical setting. Here we focus on the isolation, expansion, and myocardial differentiation of rat CPCs. Brief adaptations of the protocol for isolation from mouse and human tissue are also provided.