Slitflow: a Python framework for single-molecule dynamics and localization analysis
Slitflow: a Python framework for single-molecule dynamics and localization analysis
复制标题
Slitflow:用于单分子动力学和定位分析的 Python 框架
DOI:
10.1101/2023.03.01.530718
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发表时间:
2023
期刊:
影响因子:
--
通讯作者:
Tokunaga Makio
中科院分区:
文献类型:
--
作者:
Ito Yuma;Hirose Masanori;Tokunaga Makio
Single-molecule imaging is a promising method for direct quantification of the dynamics and distribution of biomolecules in living cells. Although numerous methods have been developed to gain biological insights into molecular behavior, the high diversity of microscopes and single-molecule dynamics can result in incomplete reproducibility of analyses. Here, we present Slitflow, an open-source framework for a single-molecule analysis workflow that includes image processing, dynamics analysis, and figure creation. We demonstrated the integrity and flexibility of the workflow using (1) a cherry-picked tracking method combining popular tools and (2) various state-of-the-art analyses in a single pipeline. The software accommodates a large variety of data and methods, paving the way for integrative analyses.
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