RADIATION RESPONSE OF MAMMALIAN CELLS CROWN IN CULTURE .V. TEMPERATURE DEPENDENCE OF REPAIR OF X-RAY DAMAGE IN SURVIVING CELLS ( AEROBIC AND HYPOXIC )
RADIATION RESPONSE OF MAMMALIAN CELLS CROWN IN CULTURE .V. TEMPERATURE DEPENDENCE OF REPAIR OF X-RAY DAMAGE IN SURVIVING CELLS ( AEROBIC AND HYPOXIC )
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DOI：
10.2307/3571978
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发表时间：
1965-01-01
影响因子：
3.4
通讯作者：
SWAIN, RW
中科院分区：
文献类型：
--
作者：
ELKIND, MM;SUTTONGI.H;SWAIN, RW
研究了培养的哺乳动物细胞亚致死x射线损伤修复的温度和氧依赖性。研究发现，存活的细胞在室温下可以相当有效地修复损伤，甚至在1℃时也能进行一些修复。此外，修复过程不需要氧气。此外，缺氧细胞可以在室温下修复亚致死损伤。由于哺乳动物细胞的生长和分裂能力强烈依赖于温度，而损伤的修复不依赖于温度，从这些结果我们已经能够表明，在最佳生长温度下，存活细胞的分次剂量反应由两个同时发生的过程组成。修复过程导致净存活率迅速增加。当细胞在不同敏感性的反应状态下进行修复时，细胞进展过程导致净存活的振荡。
The temperature and oxygen dependence of the repair of sublethal X-ray damage in cultured mammalian cells has been examined. It was found that surviving cells can repair damage quite effectively at room temperature, and some repair is effected even at 1[degree]C. Also, the repair process does not require oxygen. Moreover, hypoxic cells can repair sublethal damage at room temperature. Since the ability of mammalian cells to grow and divide is strongly temperature dependent and repair of damage is not, from these results we have been able to show that the fractionated dose response of surviving cells at an optimal growth temperature consists of 2 simultaneous processes. Repair processes lead to a prompt increase in net survival. Cell progression processes lead to oscillations in net survival as cells undergoing repair move through response states of different sensitivities.