FUNCTIONAL EQUIVALENCE OF TOP COMPONENT A RNA AND COAT PROTEIN IN INITIATION OF INFECTION BY ALFALFA MOSAIC VIRUS
FUNCTIONAL EQUIVALENCE OF TOP COMPONENT A RNA AND COAT PROTEIN IN INITIATION OF INFECTION BY ALFALFA MOSAIC VIRUS
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DOI：
10.1016/0042-6822(71)90007-9
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发表时间：
1971-01-01
期刊：
影响因子：
3.7
通讯作者：
JASPARS, EMJ
中科院分区：
文献类型：
--
作者：
BOL, JF;VANVLOTE.L;JASPARS, EMJ
通过区带梯度离心纯化苜蓿花叶病毒（AMV）的核蛋白，并通过凝胶电泳确定其均质性和RNA含量。每个成分都含有一种特定长度的 RNA。仅当存在底部成分 (B)、中间成分 (M) 和顶部成分 b (Tb) 核蛋白时，纯化制剂的组合才具有感染性。然而，在三种相应 RNA 的混合物中没有发现感染性。通过向该混合物中添加第四种 RNA，即顶部成分 aRNA (Ta-RNA)，可以开始感染。 B、M 和 Tb 核蛋白的混合物具有感染性，而相应的核酸混合物则不具有感染性，这一事实可以通过发现后者在添加少量 AMV 外壳蛋白后变得具有感染性来解释。有证据表明，AMV 外壳蛋白的活性并不是由于保护 RNA 免受降解酶的影响。讨论了 Ta-RNA 及其翻译产物（外壳蛋白）在感染发生时的功能。
The nucleoproteins of alfalfa mosaic virus (AMV) were purified by zonal gradient centrifugation, and their homogeneity and RNA contents were established by gel electrophoresis. Each component was shown to contain one type of RNA of a specific length. Combinations of purified preparations were infectious only when the bottom component (B), middle component (M), and the top componentb(Tb) nucleoproteins were present. However, no infectivity was found in a mixture of the three corresponding RNAs. Infection could be started by adding to this mixture a fourth RNA, the top componentaRNA (Ta-RNA). The fact that a mixture of B, M, and Tb nucleoprotein is infectious while the corresponding nucleic acid mixture is not, is explained by the finding that the latter becomes infectious upon addition of low amounts of AMV coat protein. Evidence is presented that the activity of AMV coat protein is not due to protection of the RNAs against degrading enzymes. The function of Ta-RNA and its translation product—the coat protein—in the onset of infection is discussed.