Specificity of cytotoxic T cells from athymic mice.
Specificity of cytotoxic T cells from athymic mice.
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DOI：
10.1084/jem.152.3.688
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发表时间：
1980-09-01
期刊：
影响因子：
--
通讯作者：
Bevan MJ
中科院分区：
文献类型：
--
作者：
Hünig T;Bevan MJ
在正常小鼠中，胸腺中的自身H-2抗原对T细胞特异性具有深远的影响。因此，我们研究了来自无胸腺裸鼠的细胞毒性T淋巴细胞（CTL）前体的特性（5），认为它们可能为研究受体特异性更接近于种系编码库的T细胞提供模型系统。发现裸CTL的前体细胞本身是THy-1+细胞。这些裸t细胞通过胎盘转移来自表型正常母亲的可能性被排除。在T细胞生长因子的存在下，裸CTL可以诱导多克隆激活伴刀豆球蛋白A或刺激同种异体或三硝基苯基（TNP）修饰的同系刺激细胞，但不是由刺激与少数H抗原的上下文中的自我H-2。同种异体反应性裸CTL--像来自正常小鼠的那些--在免疫细胞-靶细胞相互作用中识别H-2K-和H-2D-区编码的抗原，但是，与正常CTL不同，不与第三方靶细胞交叉反应。而裸鼠的抗TNP反应是H-2限制性的，它似乎不受自身H-2抗原的影响，以相同的方式在正常小鼠。这一发现表明，在正常（H-2d X H-2b）F1小鼠的抗TNP自身反应中，H-2k相对于H-2d的免疫优势在（H-2d X H-2k）F1裸鼠中不存在。这些观察结果进行了讨论，在正常的成熟T细胞受体库的产生中的胸腺的作用。
In normal mice, self-H-2 antigens in the thymus have a profound influence on T cell specificity. We have therefore investigated the properties of cytotoxic T lymphocyte (CTL) precursors from athymic nude mice (5) with the notion that they may provide a model system for the study of T cells whose receptro specificity is closer to the germ-line- encoded repertoire. It was found that the precursors of nude CTL are, themselves, THy-1+ cells. The possibility that these nude t cells were derived from the phenotypically normal mother by placental transfer was ruled out. In the presence of T cell growth factor, nude CTL can be induced by polyclonal activation with concanavalin A or by stimulation with allogeneic or trinitrophenyl (TNP)-modified syngeneic stimulator cells, but not by stimulation with minor H antigens in the context of self-H-2. Alloreactive, nude CTL--like those from normal mice-- recognize H-2K- and H-2D-region-encoded antigens in killer-target cell interactions, but, unlike normal CTL, did not cross-react on third- party target cells. Whereas the anti-TNP response of nude mice is H-2 restricted, it does not seem to be influenced by self-H-2 antigens in the same manner as in normal mice. This is suggested by the finding that the immunodominance of H-2k over H-2d in the anti-TNP-self response of normal (H-2d X H-2b)F1 mice is absent in (H-2d X H-2k)F1 nude mice. These observations are discussed in relation to the role of the thymus in the generation of the normal mature T cell receptor repertoire.