The predominant Th1 cytokine profile in maternal plasma of preeclamptic women is not reflected in the choriodecidual and fetal compartments
The predominant Th1 cytokine profile in maternal plasma of preeclamptic women is not reflected in the choriodecidual and fetal compartments
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DOI：
10.1016/j.jsgi.2005.02.005
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发表时间：
2005-07-01
期刊：
影响因子：
--
通讯作者：
Hernandez-Guerrero, C
中科院分区：
文献类型：
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作者：
Arriaga-Pizano, L;Jimenez-Zamudio, L;Hernandez-Guerrero, C
目的：人类妊娠疾病，如先兆子痫，被认为涉及细胞因子水平的变化。已经提出，在先兆子痫妇女中，有利于Th 1型细胞因子谱的平衡决定了对妊娠的局部或全身免疫应答，这可能导致有缺陷的胎盘植入和胎盘缺血，从而激活全身内皮细胞。本研究的目的是确定细胞因子的表达是否在母体、绒毛膜蜕膜和胎儿间室以及有或无先兆子痫的妇女之间存在差异。干扰素γ的血浆浓度通过酶联免疫吸附测定（ELISA）测量从母体外周血（MPB）、绒毛膜蜕膜（CD）、和胎儿脐带血（FC）室的17名妇女与先兆子痫和15名血压正常的妇女。流式细胞仪测定T淋巴细胞内IFN γ和IL-2的浓度。结果：子痫前期患者血浆中MPB和CD区室的IFN γ浓度显著高于血压正常的妇女。子痫前期孕妇血浆IL-4浓度显著低于血压正常妇女。细胞内IFN-γ和IL-2浓度没有显着差异先兆子痫和血压正常women.CONCLUSIONS：占主导地位的Th 1型Th 2型细胞因子的配置文件是明显的MPB，但暴动的CD和FC血室。这可能反映了胎儿-胎盘界面中复杂的细胞因子网络，可能涉及滋养层细胞或蜕膜和内皮细胞，这可能是血浆IFN γ浓度和辅助性T细胞数量增加的原因。版权所有（c）2005年由妇科调查协会。
OBJECTIVE: Human pregnancy disorders such as preeclampsia arc thought to involve variations in cytokine levels. It has been proposed that, in preeclamptic women, a balance favoring the Th1-type over the Th2-type cytokine profile determines local or systemic immunologic responses to pregnancy and that this may cause defective placental implantation and placental ischemia, which activate systemic endothelial cells. The purpose of this study was to determine whether cytokine expression differs in the maternal, choriodecidual, and fetal compartments, and between women with or without preeclampsia.METHODS: Plasma concentrations of interferon gamma (IFN gamma), interleukin (IL)-2, IL-4, and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA) in samples obtained from maternal peripheral blood (MPB), choriodecidual (CD), and fetal cord (FC) blood compartments of 17 women with preeclampsia and in 15 normotensive women. Intracellular concentrations of IFN gamma and IL-2 in T lymphocytes were assessed by flow cytometry.RESULTS: Plasma IFN gamma concentrations in both MPB and CD compartments were significantly higher in preeclamptic than in normotensive women. Maternal plasma IL-4 concentration was significantly lower in preeclamptic than in normotensive women. Intracellular IFN gamma and IL-2 concentrations did not differ significantly between preeclamptic and normotensive women.CONCLUSIONS: The dominant Th1-type over Th2-type cytokine profile is evident in MPB, but riot in the CD and FC blood compartments. This might reflect the complex cytokine networks in the fetal-placental interface and might involve trophoblasts or decidual and endothelial cells, which could account for the increased plasma IFN gamma concentration and T-helper cell number. Copyright (c) 2005 by the Society for Gynecologic Investigation.