Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bloactive glass
Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bloactive glass
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DOI：
10.1016/j.biomaterials.2007.05.038
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发表时间：
2007-10-01
期刊：
影响因子：
14
通讯作者：
Ducheyne, Paul
中科院分区：
文献类型：
--
作者：
Reilly, Gwendolen C.;Radin, Shula;Ducheyne, Paul
生物活性玻璃既可以作为骨填充物，也可以作为植入物的涂层，被认为是一种潜在的组织工程成骨支架。与标准的组织培养塑料相比，大鼠来源的间充质干细胞(MSCs)在45S5生物活性玻璃上生长时显示出更高的碱性磷酸酶活性水平。同样，暴露在45S5的溶解产物中会提高这些细胞中碱性磷酸酶的活性和其他成骨标志物。我们研究了在与大鼠MSCs相同的实验室条件下生长的人MSCs是否会表现出类似的反应。一般来说，无论细胞培养条件如何，人MSCs产生的碱性磷酸酶活性都明显低于大鼠MSCs，并且对生长因子BMP-2的反应方式与大鼠MSCs不同。在我们的实验中，生长在45S5生物活性玻璃或组织培养塑料上的人MSCs在来自五个不同骨科患者的样本中碱性磷酸酶活性没有差异，与培养基组分无关。45S5溶出物对三种不同供者来源的人MSCs的作用也不一致。这些结果表明，生物活性玻璃对人类患者骨生长的积极作用并不是通过促进间充质干细胞的分化来实现的。(C)2007爱思唯尔有限公司。保留所有权利。
Bioactive glass is used as both a bone filler and as a coating on implants, and has been advocated as a potential osteogenic scaffold for tissue engineering. Rat-derived mesenchymal stem cells (MSCs) show elevated levels of alkaline phosphatase activity when grown on 45S5 bioactive glass as compared to standard tissue culture plastic. Similarly, exposure to the dissolution products of 45S5 elevates alkaline phosphatase activity and other osteogenic markers in these cells. We investigated whether human MSCs grown under the same laboratory conditions as rat MSCs would exhibit similar responses. In general, human MSCs produce markedly less alkaline phosphatase activity than rat MSCs, regardless of cell culture conditions, and do not respond to the growth factor BMP-2 in the same way as rat MSCs. In our experiments there was no difference in alkaline phosphatase activity between human MSCs grown on 45S5 bioactive glass or tissue culture plastic, in samples from five different orthopaedic patients, regardless of culture media composition. Neither was there any consistent effect of 45S5 dissolution products on human MSCs from three different donors. These results suggest that the positive effects of bioactive glass on bone growth in human patients are not mediated by accelerated differentiation of mesenchymal stem cells. (c) 2007 Elsevier Ltd. All rights reserved.