Placental telomere length decreases with gestational age and is influenced by parity: A study of third trimester live-born twins
Placental telomere length decreases with gestational age and is influenced by parity: A study of third trimester live-born twins
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DOI：
10.1016/j.placenta.2014.05.010
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发表时间：
2014-10-01
期刊：
影响因子：
3.8
通讯作者：
Zeegers, M. P.
中科院分区：
文献类型：
--
作者：
Gielen, M.;Hageman, G.;Zeegers, M. P.
背景：与出生后相比，人们对出生前端粒长度知之甚少。尽管子宫内生长迟缓和先兆子痫与较短的胎盘TL相关，但胎盘TL的减少仍不清楚。本研究的目的是评估减少胎盘TL在妊娠晚期，并探讨潜在的“生长影响因素”的作用。方法：研究样本包括329个活产双胞胎从东弗兰德斯前瞻性双胞胎调查。使用多重定量PCR方法测定TL。孕龄、性别、胎次、胎盘特征、产次、母亲和父亲年龄、糖尿病、高血压、吸烟、饮酒和社会经济地位（SES）被认为是“生长影响因素”。结果：胎盘总长度范围为4.3 ~ 84.4kbp，中位数为10.8kbp。Ln（TL）以线性方式下降，估计的TL从28周的13.98 kbp下降到42周的10.56 kbp。与社会经济地位综合考虑，胎龄的回归系数变小（B = -0.017; p = 0.08）或糖尿病（B = -0.018; p = 0.07），如果与产次一起考虑，则更大（B = -0.022; p = 0.02），表明孕龄和端粒长度之间的部分关联可以通过这三个混杂因素来解释。活产双胞胎妊娠晚期胎盘TL减少约25%，表明端粒缩短可能在胎盘老化中起作用。(C)2014爱思唯尔有限公司版权所有。
Background: In contrast to the postnatal period, little is known about telomere length (TL) during prenatal life. The decrease in placental TL remains unknown, although intra uterine growth retardation and preeclampsia are associated with shorter placental TL. The aim of this study is to assess the decrease of placental TL during the third trimester of gestation and to explore the role of potential "growth influencing factors".Methods: The study sample consisted of 329 live-born twins from the East Flanders Prospective Twin Survey. TL was determined using a multiplex quantitative PCR method. Gestational age, sex, birth order, placental characteristics, parity, maternal and paternal age, diabetes, hypertension, smoking, alcohol use, and socio economic status (SES) were considered "growth influencing factors". Bivariable multilevel regression analysis with "growth influencing factors" was performed.Results: Placental TL ranged from 4.3 kbp to 84.4 kbp with a median of 10.8 kbp. Ln(TL) decreased in a linear fashion with an estimated TL decreasing from 13.98 kbp at 28 weeks to 10.56 kbp at 42 weeks. The regression coefficient of gestational age became smaller if considered together with SES (b = -0.017; p = 0.08) or diabetes (b = -0.018; p = 0.07) and bigger if considered together with parity (b = -0.022; p = 0.02), indicating that part of the association between gestational age and telomere length is explained by these three confounding factors.Conclusion: Placental TL decreases during the third trimester of gestation of live-born twins with approximately 25% indicating that telomere shortening may play a role in aging of the placenta. (C) 2014 Elsevier Ltd. All rights reserved.