Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua
Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua
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DOI：
10.1016/j.mimet.2011.01.027
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发表时间：
2011-05-01
影响因子：
2.2
通讯作者：
Moller, Simon G.
中科院分区：
文献类型：
--
作者：
Lovdal, Trond;Hovda, Maria Befring;Moller, Simon G.
与平板计数和共聚焦荧光显微镜相比，单叠氮丙啶（PMA）和定量实时PCR（qPCR）的组合显著高估了活无害李斯特菌的分数。我们的数据表明，必须谨慎使用PMA-qPCR作为区分活菌和死菌的分析工具。(C)2011 Elsevier B. V.保留所有权利。
The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria. (C) 2011 Elsevier B.V. All rights reserved.