The detection method for small molecules coupled with a molecularly imprinted polymer/quantum dot chip using a home-built optical system
The detection method for small molecules coupled with a molecularly imprinted polymer/quantum dot chip using a home-built optical system
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DOI：
10.1007/s00216-016-9620-y
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发表时间：
2016-05
影响因子：
4.3
通讯作者：
Yixi Liu;Yong Wang;Le Liu;Yonghong He;Qinghua He;Y. Ji
中科院分区：
文献类型：
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作者：
Yixi Liu;Yong Wang;Le Liu;Yonghong He;Qinghua He;Y. Ji
本研究首次提出了一种利用自制光学流体系统，利用分子印迹聚合物/量子点（MIP-QD）芯片检测小分子的方法。以莱克多巴胺（Ractopamine， RAC）为模型分子验证了其可行性。目标分子的传感是基于量子点的猝灭量。该方法简便，节省成本，易于小型化，避免了使用荧光光谱仪获得荧光猝灭曲线所需的繁琐步骤。最重要的是，使用该方法可以获得更多的细节和准确的响应时间。实验结果表明，制备的芯片具有低成本的高选择性，自制的检测系统可以实现快速的结合动力学。利用记录的猝灭过程研究了RAC在MIP-QD芯片上的吸附动力学和对RAC的特异性。该体系可进一步用于研究溶剂、pH和温度对制备的MIP选择性的影响。这种方法可以推广到其他类似的不同分子的研究中。图示：分子印迹聚合物/量子点芯片捕获目标分子的示意图
A method to detect small molecules with a molecularly imprinted polymer/quantum dot (MIP-QD) chip using a home-built optical fluidic system was first proposed in this study. Ractopamine (RAC) was used as the model molecule to demonstrate its feasibility. The sensing of the target molecule is based on the quenching amount of the quantum dots. The method is facile, cost-saving, easy for miniaturization and avoids the cumbersome steps that are needed to get the fluorescent quenching curve using a spectrofluorometer. Most importantly, more details and accurate response time can be obtained by use of this method. The experimental results show that the prepared chips with low cost are highly selective and the home-built detection system allows the fast binding kinetics. The recorded quenching process was used to study the kinetic uptake of RAC onto the MIP-QD chip and the specificity towards RAC. The system can further be utilized to study the effect of the solvent, pH and temperature on the selectivity of the prepared MIP. The methodology could be extended to other similar studies with different molecules.Graphical abstractSchematic illustration of the molecularly imprinted polymer/quantum dot chip capturing the target molecule