DNA methylation pattern in human zygotes and developing embryos
DNA methylation pattern in human zygotes and developing embryos
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DOI：
10.1530/rep.1.00217
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发表时间：
2004-12-01
期刊：
影响因子：
3.8
通讯作者：
Fulka, J
中科院分区：
文献类型：
--
作者：
Fulka, H;Mrazek, M;Fulka, J
我们报告的全球甲基化/去甲基化模式的观察人类受精卵和早期胚胎的囊胚期。我们的研究结果表明，在大约一半的受精卵检查父亲的染色质甲基化低于母亲的染色质。在另一半，两个原核显示出相同的标记强度。在发育中的胚胎的细胞核被强烈标记的四细胞阶段，此后，标记强度下降被检测到。某些细胞核的再甲基化开始于桑椹胚晚期。令人惊讶的是，与小鼠不同，在胚泡阶段，内细胞团显示出比滋养外胚层细胞更弱的标记强度。
We report on observations of the global methylation/demethylation pattern of both pronuclei in human zygotes and in early embryos up to the blastocyst stage. Our results demonstrate that in about half of the zygotes examined the paternal chromatin was less methylated than the maternal chromatin. In the other half, both pronuclei exhibited the same intensity of labeling. The nuclei in developing embryos were intensively labeled for up to the four-cell stage; thereafter, a decline of labeling intensity was detected. Remethylation in some nuclei starts in late morulae. Surprisingly, and unlike the mouse, at the blastocyst stage the inner cell mass showed a weaker intensity of labeling than the trophectodermal cells.