THE BINDING OF FATTY ACIDS BY SERUM ALBUMIN, A PROTECTIVE GROWTH FACTOR IN BACTERIOLOGICAL MEDIA
THE BINDING OF FATTY ACIDS BY SERUM ALBUMIN, A PROTECTIVE GROWTH FACTOR IN BACTERIOLOGICAL MEDIA
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DOI：
10.1084/jem.86.3.215
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发表时间：
1947-01-01
影响因子：
15.3
通讯作者：
DUBOS, RJ
中科院分区：
文献类型：
--
作者：
DAVIS, BD;DUBOS, RJ
血清白蛋白通过在培养液中结合微量的脂肪酸，允许通过接种最少的结核杆菌来启动生长。一个白蛋白分子与3到6个油酸分子结合得足够紧密，以防止细菌抑制。这种特性需要天然的白蛋白。许多其他蛋白质没有能力结合油酸；结晶的B-乳球蛋白可以结合一些。白蛋白的结合能力对其在动物体内的生理意义(运输；对细胞毒素的保护)和蛋白质的结构的意义进行了讨论。吐温80(聚氧乙烯山梨醇单油酸酯)对小接种菌的抑制作用是由于其含有可去除的未酯化油酸(质量分数为0.6%)所致。
Serum albumin, by binding traces of fatty acid in the media, permits initiation of growth by minimal inocula of tubercle bacilli. A molecule of albumin binds 3 to 6 molecules of oleic acid tightly enough to prevent bacteriostasis. The property requires unde-natured albumin. Many other proteins have no capacity to bind oleic acid; crystalline B-lactoglobulin binds some. The implications of the binding capacity of albumin for its physiological significance in the animal body (transport; protection against cytotoxins), and for the structure of the protein, are discussed. The inhibitory effect of Tween 80 (polyoxy-ethylene sorbitan monooleate) on small inocula of tubercle bacilli is caused by its content of unesterified oleic acid (0.6% by wt.), which can be removed.