Growth inhibitory and bactericidal activities of lansoprazole compared with those of omeprazole and pantoprazole against Helicobacter pylori
Growth inhibitory and bactericidal activities of lansoprazole compared with those of omeprazole and pantoprazole against Helicobacter pylori
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DOI：
10.1046/j.1523-5378.1998.08024.x
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发表时间：
1998-03-01
期刊：
影响因子：
4.4
通讯作者：
Malfertheiner, P
中科院分区：
文献类型：
--
作者：
Nakao, M;Malfertheiner, P
背景幽门螺杆菌在十二指肠溃疡和胃溃疡的发病机制中起作用。本研究的目的是评价质子泵抑制剂（PPI）兰索拉唑（常用于根除方案）对H. pylori in vitro comparing with other PPI.材料和方法通过使用琼脂稀释法测定最小抑制浓度，评价每种PPI的生长抑制活性。通过分析与不同浓度PPI孵育后不同时间点培养物中的活细胞来测定杀菌活性。在暴露于供试品后，使用固定细胞的扫描电子显微镜检查细菌形态。尿素酶活性测定结果表明，H.用比色法测定与递增浓度PPI孵育的pylori的浓度。兰索拉唑的生长抑制活性显著强于奥美拉唑或泮托拉唑（MIC 90分别为6.25 vs. 25和100 μ g/ml）。暴露H。pylori感染后，兰索拉唑导致存活力丧失和细菌形态异常，其范围比奥美拉唑或泮托拉唑更广。兰索拉唑剂量依赖性地抑制尿素酶活性;其有效性与奥美拉唑相当，但比泮托拉唑更有效。对导致H.研究的三种PPI之间的幽门螺杆菌细胞似乎不同;兰索拉唑在生长抑制和细菌形态破坏方面是三种药物中最有效的。
Background. Helicobacter pylori plays a role in the pathogenesis of both duodenal and gastric ulcers. The aim of this study was to evaluate the effect of the proton pump inhibitor (PPI), lansoprazole, commonly used in eradication regimens, on growth, bactericidal activity and morphology of H. pylori in vitro in comparison with other PPIs.Materials and Methods. Growth inhibitory activity of each of the PPIs was evaluated by determining minimum inhibitory concentrations using an agar dilution method. Bactericidal activity was determined by analysis of the viable cells in culture at various time points after incubation with different concentrations of the PPIs. Bacterial morphology was examined using scanning electron microscopy of fixed cells after exposure to the test substances. Urease activity in cell extracts of H. pylori that had been incubated with increasing concentrations of the PPIs was determined by colorimetry.Results. The growth inhibitory activity of lansoprazole was significantly more potent than that of omeprazole or pantoprazole (MIC90 6.25 vs. 25 and 100 mu g/ml, respectively). Exposure of H. pylori to lansoprazole produced loss of viability and an aberrant bacterial morphology, which was more extensive than seen with omeprazole or pantoprazole. Lansoprazole dose dependently inhibited urease activity; its effectiveness was comparable with omeprazole but more potent than pantoprazole.Conclusions. The mechanism of action that leads to loss of viability of H. pylori cells appears to differ between the three PPIs investigated; lansoprazole was the most potent of the three agents in terms of growth inhibition and disruption of bacterial morphology.