Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model
Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model
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DOI：
10.1292/jvms.12-0448
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发表时间：
2013-04-01
影响因子：
1.2
通讯作者：
Mizuno, Takuya
中科院分区：
文献类型：
--
作者：
Umeki, Saori;Ema, Yasuo;Mizuno, Takuya
从犬淋巴瘤患者身上建立了5种新型犬淋巴瘤细胞系（Ema、CLC、CLK、Nody-1和UL-1），并在体外和体内进行了鉴定。通过流式细胞分析和抗原受体重排聚合酶链反应，除CLC外，所有细胞系均具有t细胞表型。细胞增殖率和MYC、PTEN、KIT和FLT3的转录水平在不同细胞系之间存在差异。将Ema、CLC、NOD -1和UL-1淋巴瘤细胞系腹腔异种移植到NOD/SCID小鼠体内，可引起腹水、腹膜内肿瘤和严重的淋巴瘤细胞浸润到胰腺和肠系膜。建立具有不同特征的新型犬淋巴瘤细胞系对于阐明犬淋巴瘤的病理生理和改进现有的治疗方法至关重要。
Five novel, canine lymphoma cell lines (Ema, CLC, CLK, Nody-1 and UL-1) were established from dogs suffering from lymphoma and characterized in vitro and in vivo. All cell lines, except CLC, were characterized with T-cell phenotypes, by flow cytometric analysis and polymerase chain reaction for antigen receptor rearrangement. Cell proliferation rates and transcriptional levels of MYC, PTEN, KIT and FLT3 varied between each cell line. Intraperitoneal xenotransplantation of Ema, CLC, Nody-1 and UL-1 lymphoma cell lines into NOD/SCID mice induced ascites, intraperitoneal tumors and severe infiltration of lymphoma cells into the pancreas and mesentery. Establishment of novel canine lymphoma cell lines with different characteristics is critical for elucidating the pathophysiology of canine lymphoma and improving current therapies.