ANKHD1, ankyrin repeat and KH domain containing 1, is overexpressed in acute leukemias and is associated with SHP2 in K562 cells
ANKHD1, ankyrin repeat and KH domain containing 1, is overexpressed in acute leukemias and is associated with SHP2 in K562 cells
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DOI：
10.1016/j.bbadis.2006.07.010
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发表时间：
2006-09-01
影响因子：
6.2
通讯作者：
Saad, Sara T. O.
中科院分区：
文献类型：
--
作者：
Traina, Fabiola;Favaro, Patricia M. B.;Saad, Sara T. O.
在本研究中，与正常造血细胞相比，白血病细胞系中ANKHD 1 mRNA和蛋白表达水平增加。ANKHD 1 mRNA在急性白血病细胞中的表达明显高于正常造血细胞（P=0.002）。ANKHD 1在K562和LNCaP细胞系的蛋白提取物中与SHP 2共免疫沉淀。这些发现表明ANKHD 1作为支架蛋白的作用可能与白血病细胞的异常表型有关。(c)2006 Elsevier B.V.保留所有权利。
In the present study, increased levels of ANKHD1 mRNA and protein expression in leukemia cell lines are reported, as compared with normal hematopoietic cells. Furthermore, a higher expression of ANKHD1 mRNA was detected in primary acute leukemia samples than in normal hematopoietic cells (P=0.002). ANKHD1 was detected in the cytosolic and membrane fraction of cells and was co-immumoprecipitated with SHP2 in protein extracts of K562 and LNCaP cell lines. These findings suggest a role for ANKHD1 as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. (c) 2006 Elsevier B.V. All rights reserved.