Restricted expression of Neuroglobin in the mouse retina and co-localization with Melanopsin and Tyrosine Hydroxylase
Restricted expression of Neuroglobin in the mouse retina and co-localization with Melanopsin and Tyrosine Hydroxylase
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DOI：
10.1016/j.bbrc.2012.07.061
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发表时间：
2012-08-17
影响因子：
3.1
通讯作者：
Hannibal, J.
中科院分区：
文献类型：
--
作者：
Hundahl, C. A.;Fahrenkrug, J.;Hannibal, J.
脑红蛋白(NGB)是一种神经元特异性氧结合血红素-珠蛋白，据报道在小鼠视网膜的大部分层中都有高水平的表达。NGB的功能目前尚不清楚，但基于其高表达水平和氧结合能力，NGB被认为是促进高活性神经元氧代谢的氧库或神经保护剂。在本研究中，我们使用一种高度有效的抗体重新检测了NGB在视网膜中的表达模式。此外，还研究了NGB基因缺失小鼠视网膜-下丘脑投射的完整性以及黑素蛋白和酪氨酸羟基酶的视网膜表达水平。在神经节细胞和内核层中分别共表达黑素和酪氨酸羟基酶的少数神经元呈NGB免疫反应阳性。NGB缺乏既不影响黑素蛋白和酪氨酸羟基酶蛋白的水平，也不影响视交叉上核内PACAP阳性的视网膜下丘脑投射的完整性。根据目前的结果，在正常情况下，NGB似乎不太可能在视网膜氧稳态和神经元存活中发挥重要作用。目前的研究表明，以前发表的一些报告依赖于特异性可疑的抗体。(C)2012 Elsevier Inc.保留所有权利。
Neuroglobin (Ngb), a neuronal specific oxygen binding heme-globin, reported to be expressed at high levels in most layers of the murine retina. Ngb's function is presently unknown, but based on its high expression level and oxygen binding capabilities Ngb was proposed to function as an oxygen reservoir facilitating oxygen metabolism in highly active neurons or to function as a neuroprotectant. In the present study, we re-examined the expression pattern of Ngb in the retina using a highly validated antibody. Furthermore, intactness of retino-hypothalamic projections and the retinal expression level of Melanopsin and Tyrosine Hydroxylase were investigated in Ngb-null mice. Ngb-immunoreactivity was found in a few neurons of the ganglion cell and inner nuclear layers co-expressing Melanopsin and Tyrosine Hydroxylase, respectively. Ngb deficiency neither affected the level of Melanopsin and Tyrosine Hydroxylase proteins nor the intactness of PACAP-positive retinohypothalamic projections in the suprachiasmatic nucleus. Based on the present results, it seems unlikely that Ngb could have a major role in retinal oxygen homeostasis and neuronal survival under normal conditions. The present study suggests that a number of previously published reports have relied on antibodies with dubious specificity. (C) 2012 Elsevier Inc. All rights reserved.