Polymer-Coated Magnetic Microspheres Conjugated with Growth Factor Receptor Binding Peptides Enable Cell Sorting
Polymer-Coated Magnetic Microspheres Conjugated with Growth Factor Receptor Binding Peptides Enable Cell Sorting
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DOI：
10.1021/acsbiomaterials.1c01199
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发表时间：
2021-12-13
影响因子：
5.8
通讯作者：
Gopalan, Padma
中科院分区：
文献类型：
--
作者：
Krutty, John D.;Sun, Jian;Gopalan, Padma
人体细胞的分离和分选是细胞疗法生物加工的重要步骤。必须对异质细胞混合物进行分选，以分离所需的细胞类型并纯化最终产品。这个过程通常是通过基于抗体的分选技术来实现的。在这项工作中，我们证明磁性微球可以用肽进行功能化，这些肽根据特定表面蛋白的相对浓度选择性地与细胞结合。五微米磁性微球涂有合成共聚物PVG（聚（聚（乙二醇）甲基醚甲基丙烯酸酯-ran-乙烯基二甲基吖内酯-ran-甲基丙烯酸缩水甘油酯），并用血管内皮生长因子受体结合肽（VRBP）功能化，该肽与血管内皮生长因子受体（VEGFR）结合。这些微球表现出低细胞毒性，并根据其相对表面蛋白表达与细胞结合。涂层磁性微球用于通过磁辅助细胞分选（MACS）根据其 VEGFR 表达来分离异质细胞群，这表明基于肽的细胞分选机制可用于基于人类细胞的产品的生物加工。
The separation and sorting of human cells is an important step in the bioprocessing of cell-based therapeutics. Heterogeneous mixtures of cells must be sorted to isolate the desired cell type and purify the final product. This process is often achieved by antibody-based sorting techniques. In this work, we demonstrate that magnetic microspheres may be functionalized with peptides that selectively bind to cells on the basis of their relative concentration of specific surface proteins. Five-micrometer-magnetic microspheres were coated with the synthetic copolymer PVG (poly(poly(ethylene glycol)methyl ether methacrylate-ran-vinyl dimethyl azlactone-ran-glycidyl methacrylate) and functionalized with the vascular endothelial growth factor receptor binding peptide (VRBP), which binds to the vascular endothelial growth factor receptor (VEGFR). These microspheres exhibited low cytotoxicity and bind to cells depending on their relative surface protein expression. Finally, coated, magnetic microspheres were used to separate heterogeneous populations of cells dependent on their VEGFR expression through magnetic-assisted cell sorting (MACS), demonstrating that peptide-based cell sorting mechanisms may be useful in the bioprocessing of human-cell-based products.