CULTURAL METHODS AND SOME CHARACTERISTICS OF SOME OF THE MORE NUMEROUS GROUPS OF BACTERIA IN THE BOVINE RUMEN
CULTURAL METHODS AND SOME CHARACTERISTICS OF SOME OF THE MORE NUMEROUS GROUPS OF BACTERIA IN THE BOVINE RUMEN
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DOI：
10.3168/jds.s0022-0302(53)91482-9
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发表时间：
1953-01-01
影响因子：
3.5
通讯作者：
BURKEY, LA
中科院分区：
文献类型：
--
作者：
BRYANT, MP;BURKEY, LA
将瘤胃内容物样品在厌氧矿物质稀释溶液中稀释，在Waring混合器中混合以分散细菌，并在含有矿物质、瘤胃液、葡萄糖、纤维二糖、半胱氨酸和H2 CO 3-NaHCO 3缓冲系统的厌氧琼脂培养基中培养。变形，对分离出的896株细菌中的每一株进行了革兰氏反应、运动性、与O2的关系以及产生H2S、液化明胶、水解淀粉和纤维素以及从葡萄糖、木糖和纤维二糖产生酸的能力进行了测定。正常口粮动物的菌落计数平均值。1.7每毫升十亿个细菌，占细菌总数的8.4%。直接计数。除少数兼性厌氧链球菌外，所有培养的细菌均为厌氧菌。分离出的许多细菌群需要碳酸氢盐才能生长，有几种细菌除非在培养基中加入瘤胃液，否则生长不好。
Samples of rumen contents were diluted in an anaerobic mineral dilution soln., mixed in a Waring blendor to disperse the bacteria, and cultured in an anaerobic agar culture medium containing minerals, rumen fluid, glucose, cellobiose, cysteine, and H2CO3-NaHCO3 buffer system. The morph., Gram reaction, motility, relations to O2, and ability to produce H2S, liquefy gelatin, hydrolyze starch and cellulose and to produce acid from glucose, xylose, and cellobiose were determined for each of 896 strains of bacteria isolated. Colony counts from animals on normal rations avg. 1.7 billion bacteria per ml., or 8.4% of the total bacteria as detd. by direct counts. All bacteria cultured were anaerobes except for a few strains of facultative anaerobic Streptococci. Many of the groups of bacteria isolated required bicarbonate for growth and several did not grow well unless rumen fluid was included in the medium.