Roughened silver electrodes for use in metal-enhanced fluorescence
Roughened silver electrodes for use in metal-enhanced fluorescence
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DOI：
10.1016/j.saa.2003.10.014
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发表时间：
2004-07-01
影响因子：
4.4
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
Geddes, CD;Parfenov, A;Lakowicz, JR
粗糙银电极被广泛用于表面增强拉曼散射(SERS)。我们测试了粗糙的银电极的金属增强荧光。在纯水中，银电极之间的恒定电流导致了阴极上的类分形结构的生长。该电极涂覆了单层人血清白蛋白(HSA)蛋白，并用荧光染料吲哚青绿(ICG)标记。ICG-HSA在粗糙的电极上的荧光强度比未粗糙的电极增加了大约50倍，基本上是非荧光的，与银阳极相比通常增加了两倍。在阳极上没有观察到类分形结构。寿命测量表明，强度增加的至少部分原因是ICG的辐射衰减率增加。使用原位生成的粗糙银电极将在分析化学中得到广泛的应用，例如在基于荧光的分析中，类似于现在广泛使用的表面增强拉曼光谱。据我们所知，这是金属增强荧光粗糙银电极的首次报道。(C)2003爱思唯尔B.V.保留所有权利。
Roughened silver electrodes are widely used for surface-enhanced Raman scattering (SERS). We tested roughened silver electrodes for metal-enhanced fluorescence. Constant current between two silver electrodes in pure water resulted in the growth of fractal-like structures on the cathode. This electrode was coated with a monolayer of human serum albumin (HSA) protein that had been labeled with a fluorescent dye, indocyanine green (ICG). The fluorescence intensity of ICG-HSA on the roughened electrode increased by approximate to50-fold relative to the unroughened electrode, which was essentially non-fluorescent and increased typically two-fold as compared to the silver anode. No fractal-like structures were observed on the anode. Lifetime measurements showed that at least part of the increased intensity was due to an increased radiative decay rate of ICG.In our opinion. the use of in situ generated roughened silver electrodes will find multifarious applications in analytical chemistry, such as in fluorescence based assays, in an analogous manner to the now widespread use of SERS. To the best of our knowledge this is the first report of roughened silver electrodes for metal-enhanced fluorescence. (C) 2003 Elsevier B.V. All rights reserved.