Common antigenicity of mouse 42°C-specific heat-shock protein with mouse hsp 105
Common antigenicity of mouse 42°C-specific heat-shock protein with mouse hsp 105
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小鼠 42°C 特异性热休克蛋白与小鼠 hsp 105 的共同抗原性
DOI：
10.1016/0006-291x(89)91620-3
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发表时间：
1989
影响因子：
3.1
通讯作者：
M. Yukioka
中科院分区：
文献类型：
--
作者：
K. Honda;T. Hatayama;M. Yukioka
在42° C下孵育的哺乳动物细胞在42 ° C下合成特异性热休克蛋白（42° C-hsp），其不由45° C下的热休克或诱导主要热休克蛋白的其他应激诱导（Hatayama et al.（1986）Biochem. Biophys.通信资源137，957-963）。针对从小鼠FM 3A细胞纯化的分子量为105，000的热休克蛋白（hsp 105）产生的抗体与相同细胞的42° C-hsp交叉反应。该抗体仅与人HeLa细胞的hsp 105和42° C-hsp弱反应。这些结果表明，热休克蛋白105和42° C-hsp具有相同的抗原决定簇，42° C-hsp可能具有与热休克蛋白105相似的结构。
Mammalian cells incubated at 42° C synthesize a specific heat-shock protein at 42° C (42° C-hsp) that is not induced by heat-shock at 45° C or by other stresses that induce major heat shock proteins (Hatayama et al.(1986) Biochem. Biophys. Res. Commun. 137, 957–963). Antibody raised against a heat-shock protein with molecular weight of 105,000 (hsp 105) purified from mouse FM 3A cells cross-reacted to the 42° C-hsp of the same cells. The antibody reacted only weakly to hsp 105 and 42° C-hsp of human HeLa cells. These results suggested that hsp 105 and 42° C-hsp have the same antigenic determinant, and that 42° C-hsp may have a structure similar to that of hsp 105.