Long Non-coding RNA Derived from lncRNA-mRNA Co-expression Networks Modulates the Locust Phase Change.
Long Non-coding RNA Derived from lncRNA-mRNA Co-expression Networks Modulates the Locust Phase Change.
复制标题
来自 lncRNA-mRNA 共表达网络的长非编码 RNA 调节 Locust 相变。
DOI：
10.1016/j.gpb.2020.05.001
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Kang L
中科院分区：
文献类型：
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作者：
Li T;Chen B;Yang P;Wang D;Du B;Kang L
长链非编码RNA（lncRNA）调节从基因表达到动物行为的各种生物学过程。尽管蛋白质编码基因、microRNA和神经肽在飞蝗表型可塑性的调控中发挥重要作用，但关于lncRNA在这一过程中的作用的实证研究仍然有限。在这里，我们应用高通量RNA-seq来比较蝗虫相变时程中lncRNA和mRNA的表达模式。我们发现lncRNA在相变的早期阶段反应更快。功能注释表明，早期变化的lncRNA在隔离和拥挤阶段采用不同的途径来科普种群密度的变化。筛选拥挤和隔离网络中的两个重叠枢纽lncRNA基因座用于功能验证。其中LNC 1010057是一个潜在的蝗虫相变调节因子。这项工作提供了深入了解蝗虫相变的分子机制，并扩大了lncRNA在动物行为中的功能范围。
Long non-coding RNAs (lncRNAs) regulate various biological processes ranging from gene expression to animal behavior. Although protein-coding genes, microRNAs, and neuropeptides play important roles in the regulation of phenotypic plasticity in migratory locust, empirical studies on the function of lncRNAs in this process remain limited. Here, we applied high-throughput RNA-seq to compare the expression patterns of lncRNAs and mRNAs in the time course of locust phase change. We found that lncRNAs responded more rapidly at the early stages of phase transition. Functional annotations demonstrated that early changed lncRNAs employed different pathways in isolation and crowding phases to cope with changes in the population density. Two overlapping hub lncRNA loci in the crowding and isolation networks were screened for functional verification. One of them, LNC1010057, was validated as a potential regulator of locust phase change. This work offers insights into the molecular mechanism underlying locust phase change and expands the scope of lncRNA functions in animal behavior.