Direct extraction of astaxanthin from Haematococcus culture using vegetable oils
Direct extraction of astaxanthin from Haematococcus culture using vegetable oils
复制标题
DOI：
10.1007/s10529-007-9578-0
复制
发表时间：
2008-03-01
影响因子：
2.7
通讯作者：
Sim, Sang Jun
中科院分区：
文献类型：
--
作者：
Kang, Chang Duk;Sim, Sang Jun
采用一种绿色的下游工艺，使用普通植物油直接从红球藻中提取虾青素。该方法由一个单一的集成单元组成，以提取虾青素，随后分离含虾青素的油提取物。在没有细胞收获过程步骤的情况下，培养液直接与植物油混合;细胞内的虾青素通过疏水相互作用被提取到植物油相中，回收率为88%及以上。仅通过重力沉降从含有细胞碎片的培养基中简单地分离油提取物。
A green, downstream process using common vegetable oils was used for the direct extraction of astaxanthin from Haematococcus. The process consists of a single integrated unit to extract astaxanthin with subsequent separation of the astaxanthin-containing oil extract. Without a cell harvest process step, the culture broth was directly mixed with the vegetable oils; the astaxanthin inside the cell was extracted into the vegetable oil phase by hydrophobic interactions, with recovery yields of 88% and above. The oil extracts were simply separated from the culture medium containing cell debris by gravity settling only.