Antioxidant biochemical responses to long-term copper exposure in Bathymodiolus azoricus from Menez-Gwen hydrothermal vent
Antioxidant biochemical responses to long-term copper exposure in Bathymodiolus azoricus from Menez-Gwen hydrothermal vent
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DOI：
10.1016/j.scitotenv.2007.08.056
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发表时间：
2008-01-25
影响因子：
9.8
通讯作者：
Bebianno, Maria Joao
中科院分区：
文献类型：
--
作者：
Company, Rui;Serafim, Angela;Bebianno, Maria Joao
铜 (Cu) 对于海洋生物的各种生理过程至关重要。然而，在高浓度下，这种具有氧化还原活性的过渡金属可能会增强活性氧（ROS）的形成，并随后引发氧化损伤。高浓度的铜可能会增加脂质、蛋白质和 DNA 的氧化损伤。 Bathymodiolus azoricus 是一种贻贝科双壳类动物，在亚速尔群岛三重交界点附近的热液环境中非常常见，持续暴露于喷口流体散发的高浓度金属（包括铜）中。这些生物体中抗氧化防御系统和其他应激相关生物标志物的知识仍然很少。本工作的目的是研究Cu（25 μ g l(-1)；暴露24天；净化6天）对B. azoricus鳃和外套膜中抗氧化应激生物标志物的影响。应激相关生物标志物的表达具有组织依赖性，结果表明，金属暴露以外的其他因素也可能影响应激生物标志物，因为对照和铜暴露贻贝的抗氧化酶活性、MT浓度、LPO和总氧自由基清除能力（TOSC）几乎没有变化。此外，在对照贻贝和暴露于铜的贻贝中，这些参数普遍存在随时间增加的趋势，这表明活性氧（ROS）的形成不依赖于金属，并且可能与动物在不利条件下（与热液环境中相比）长期处于不利条件后的不良生理状况有关。 (c) 2007 年 Elsevier B.V. 出版
Copper (Cu) is essential to various physiological processes in marine organisms. However, at high concentrations this redox-active transition metal may enhance the formation of reactive oxygen species (ROS) and subsequently initiate oxidative damage. High concentrations of Cu may increase oxidative damage to lipids, proteins and DNA. Bathymodiolus azoricus is a Mytilid bivalve very common in hydrothermal environments near the Azores Triple Junction continuously exposed to high metal concentrations, including Cu, emanating from the vent fluids. The knowledge of antioxidant defence system and other stress related biomarkers in these organisms is still scarce. The aim of this work was to study the effect of Cu (25 mu g l(-1); 24 days exposure; 6 days depuration) on the antioxidant stress biomarkers in the gills and mantle of B. azoricus. The expression of stress related biomarkers was tissue-dependent and results suggest that other factors than metal exposure may influence stress biomarkers, since little variation in antioxidant enzymes activities, MT concentrations, LPO and total oxyradical scavenging capacity (TOSC) occurred in both control and Cu-exposed mussels. Moreover, there is a general tendency for these parameters to increase with time, in both control and Cu-exposed mussels, suggesting that reactive oxygen species (ROS) formation is not metal dependent, and may be related with poor physiological conditions of the animals after long periods in adverse conditions compared to those in hydrothermal environments. (c) 2007 Published by Elsevier B.V.