Adhesion dynamics and durotaxis in migrating cells.
Adhesion dynamics and durotaxis in migrating cells.
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DOI：
10.1088/1478-3975/8/1/015011
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发表时间：
2011-02
期刊：
影响因子：
2
通讯作者：
Sun SX
中科院分区：
文献类型：
--
作者：
Harland B;Walcott S;Sun SX
当组织细胞被电镀在柔性底物上时，已经观察到细胞向机械僵硬区域的定向迁移。环境机械信号不仅在细胞迁移中起重要作用，而且似乎影响细胞分化和发育的方方面面，包括癌细胞的转移过程。基于一个认为这种机械感觉具有力学基础的理论模型，我们引入了一个简单的细胞模型，该模型考虑了粘连运动介导的含有肌球蛋白马达(应力纤维)的F-肌动蛋白束的收缩。我们表明，当呈现一个线性的刚度梯度时，这个简单的模型表现出趋杜性。有趣的是，由于应力纤维不会在软表面上形成，而且由于粘结滑动变得与刚性表面无关，该模型预测平均细胞速度在中等刚度时达到最大值。这一预测可以在实验中得到验证。因此，我们认为，僵硬依赖的细胞适应(机械感觉)和趋向性是密切相关的，并可能共享一个力学基础。因此，我们确定了必要的物理成分，结合其他生化机制，可以解释细胞中的趋杜性和机械感觉。
When tissue cells are plated on a flexible substrate, durotaxis, the directed migration of cells towards mechanically stiff regions, has been observed. Environmental mechanical signals are not only important in cell migration, but also seem to influence all aspects of cell differentiation and development, including the metastatic process in cancer cells. Based on a theoretical model suggesting that this mechanosensation has a mechanical basis, we introduce a simple model of a cell by considering the contraction of F-actin bundles containing myosin motors (stress fibers) mediated by the movement of adhesions. We show that, when presented with a linear stiffness gradient, this simple model exhibits durotaxis. Interestingly, since stress fibers do not form on soft surfaces and since adhesion sliding becomes stiffness independent on stiff surfaces, the model predicts that the average cell velocity reaches a maximum at an intermediate stiffness. This prediction can be experimentally tested. We therefore argue that stiffness-dependent cellular adaptations (mechanosensation) and durotaxis are intimately related, and may share a mechanical basis. We therefore identify the essential physical ingredients, which combined with additional biochemical mechanisms, can explain durotaxis and mechanosensation in cells.
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影响因子：
1.6
作者：
Pelham, RJ;Wang, YL
通讯作者：
Wang, YL
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE
粘着斑蛋白在三维细胞运动中的独特作用。
影响因子：
21.3
作者：
Fraley, Stephanie I.;Feng, Yunfeng;Krishnamurthy, Ranjini;Kim, Dong-Hwee;Celedon, Alfredo;Longmore, Gregory D.;Wirtz, Denis
通讯作者：
Wirtz, Denis
影响因子：
3.7
作者：
Hale, Christopher M.;Sun, Sean X.;Wirtz, Denis
通讯作者：
Wirtz, Denis
影响因子：
3.4
作者：
Lo, CM;Wang, HB;Wang, YL
通讯作者：
Wang, YL