Members of an R2R3-MYB transcription factor family in Petunia are developmentally and environmentally regulated to control complex floral and vegetative pigmentation patterning
Members of an R2R3-MYB transcription factor family in Petunia are developmentally and environmentally regulated to control complex floral and vegetative pigmentation patterning
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DOI：
10.1111/j.1365-313x.2010.04465.x
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发表时间：
2011-03-01
期刊：
影响因子：
7.2
通讯作者：
Davies, Kevin M.
中科院分区：
文献类型：
--
作者：
Albert, Nick W.;Lewis, David H.;Davies, Kevin M.
我们提出了花色素苷调节整个矮牵牛植物的调查，确定复杂的花色素沉着模式和环境诱导的植物花色素苷合成的机制。深紫色（DPL）和紫雾（PHZ）编码调节矮牵牛花色素苷合成的R2 R3-MYB转录因子家族的成员，并控制营养组织中的花色素苷产生并有助于花色素沉着。除了这两种MYB因子，碱性螺旋-环-螺旋（bHLH）因子ANTHOCYANIN 1（AN 1）和WD-重复蛋白AN 11也是营养性色素沉着所必需的。强光诱导的花色素苷在营养组织中的诱导与PHZ和AN 1的转录本的诱导密切相关。有趣的是，转录PhMYB 27，一个假定的R2 R3-MYB活性阻遏物，在非诱导性遮荫条件下高度表达，并在强光下抑制。竞争性抑制剂PhMYBx（R3-MYB）在强光下表达，这可能提供反馈抑制。在花组织中，DPL调节花管中与脉相关的花色素苷色素沉着，而PHZ决定光诱导的花色素苷在暴露的花瓣表面上的积累（芽红）。提出了一个模型，表明如何复杂的花和营养体色素沉着模式是来自矮牵牛的MYB，bHLH和WDR共调节。
P>We present an investigation of anthocyanin regulation over the entire petunia plant, determining the mechanisms governing complex floral pigmentation patterning and environmentally induced vegetative anthocyanin synthesis. DEEP PURPLE (DPL) and PURPLE HAZE (PHZ) encode members of the R2R3-MYB transcription factor family that regulate anthocyanin synthesis in petunia, and control anthocyanin production in vegetative tissues and contribute to floral pigmentation. In addition to these two MYB factors, the basic helix-loop-helix (bHLH) factor ANTHOCYANIN1 (AN1) and WD-repeat protein AN11, are also essential for vegetative pigmentation. The induction of anthocyanins in vegetative tissues by high light was tightly correlated to the induction of transcripts for PHZ and AN1. Interestingly, transcripts for PhMYB27, a putative R2R3-MYB active repressor, were highly expressed during non-inductive shade conditions and repressed during high light. The competitive inhibitor PhMYBx (R3-MYB) was expressed under high light, which may provide feedback repression. In floral tissues DPL regulates vein-associated anthocyanin pigmentation in the flower tube, while PHZ determines light-induced anthocyanin accumulation on exposed petal surfaces (bud-blush). A model is presented suggesting how complex floral and vegetative pigmentation patterns are derived in petunia in terms of MYB, bHLH and WDR co-regulators.