Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens
Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens
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DOI：
10.1128/jcm.02097-08
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发表时间：
2009-04-01
影响因子：
9.4
通讯作者：
Parrish, Nicole
中科院分区：
文献类型：
--
作者：
Hendry, Cindy;Dionne, Kim;Parrish, Nicole
分枝杆菌病的快速检测是必不可少的。使用多种类型和浓度的分枝杆菌标本，我们比较了两种商品化的金胺O菌株。更快速的染色允许一致的抗酸杆菌定量，并显示较少的碎屑染色，染色过程需要更少的时间(类似于2分钟)。这些结果表明，快速染色在临床实验室使用可能更具成本效益和效率。
Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time (similar to 2 min) to perform. These results suggest that the rapid stain may be more cost-effective and efficient for use in clinical laboratories.