Control of lens epithelial cell survival.
Control of lens epithelial cell survival.
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DOI：
10.1083/jcb.121.4.899
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发表时间：
1993-05
影响因子：
7.8
通讯作者：
Raff, M C
中科院分区：
文献类型：
--
作者：
Ishizaki, Y;Voyvodic, J T;Burne, J F;Raff, M C
我们已经研究了发育中的透镜上皮细胞的生存要求，以验证以下假设：大多数细胞被编程为杀死自己，除非它们不断地被其他细胞告知不要这样做。透镜细胞在不存在其他细胞或添加血清或蛋白质的情况下在外植体培养物和高密度解离细胞培养物中存活数周，表明它们不需要来自其他细胞类型的信号来存活。然而，当在低密度下培养时，它们通过凋亡而死亡，这表明它们依赖于其他透镜上皮细胞存活。在琼脂糖凝胶中以高密度培养的透镜上皮细胞也存活了数周，即使它们彼此不直接接触，这表明它们可以在没有细胞-细胞接触的情况下促进彼此的存活。来自高密度培养物的条件培养基促进低密度培养的细胞的存活，表明透镜上皮细胞通过分泌存活因子来支持彼此的存活。我们第一次表明，正常的细胞死亡发生在前上皮细胞在成熟的透镜，但这种死亡是严格限制在该地区的前缝。
We have studied the survival requirements of developing lens epithelial cells to test the hypothesis that most cells are programmed to kill themselves unless they are continuously signaled by other cells not to do so. The lens cells survived for weeks in both explant cultures and high-density dissociated cell cultures in the absence of other cells or added serum or protein, suggesting that they do not require signals from other cell types to survive. When cultured at low density, however, they died by apoptosis, suggesting that they depend on other lens epithelial cells for their survival. Lens epithelial cells cultured at high density in agarose gels also survived for weeks, even though they were not in direct contact with one another, suggesting that they can promote one another's survival in the absence of cell- cell contact. Conditioned medium from high density cultures promoted the survival of cells cultured at low density, suggesting that lens epithelial cells support one another's survival by secreting survival factors. We show for the first time that normal cell death occurs within the anterior epithelium in the mature lens, but this death is strictly confined to the region of the anterior suture.