Expression of survivin, a novel inhibitor of apoptosis and cell cycle regulatory protein, in pancreatic adenocarcinoma.
Expression of survivin, a novel inhibitor of apoptosis and cell cycle regulatory protein, in pancreatic adenocarcinoma.
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DOI：
10.1038/sj.bjc.6600133
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发表时间：
2002-03-18
影响因子：
8.8
通讯作者：
Guillou, P J
中科院分区：
文献类型：
--
作者：
Sarela, A I;Verbeke, C S;Ramsdale, J;Davies, C L;Markham, A F;Guillou, P J
Survivin在人类恶性肿瘤中的表达是独一无二的，但在正常的成人细胞中并不存在。它与化疗致敏有关，并作为几种常见癌症的预后标志物。对52例胰腺和12例壶腹腺癌患者行Survivin、P53、BCL-2表达及细胞增殖指数（Ki-67）、凋亡指数（TUNEL）的免疫组化检测。46例（88%）胰腺肿瘤细胞质中检测到Survivin。P53和BCL-2分别在54%和12%的胰腺肿瘤中检测到。增殖指数为26.2±10.5%，凋亡指数为1.38±0.69%。Survivin在p53阳性组的表达率明显高于p53阴性组（P=0.05），但与BCL-2表达无相关性。Survivin表达的加权分数越高，增殖指数越高（P=0.001）。细胞增殖指数升高与细胞凋亡指数升高呈线性相关（P<0.001）。令人惊讶的是，Survivin表达越高，细胞凋亡指数越高（P=0.007）。Survivin、P53或BCL-2的表达、凋亡指数和增殖指数均不影响生存特征。壶腹癌中有83%的病例表达Survivin。然而，与胰腺癌不同的是，Survivin与P53表达或增殖指数之间没有相关性。综上所述，Survivin在大多数胰腺腺癌中表达，并与细胞增殖和凋亡相关。分子调控Survivin的表达可能会增强胰腺癌的化疗和放疗效果。英国癌症杂志（2002）86,886-892。DOI: 10.1038/sj/bjc/6600133 www.bjcancer.com©2002英国癌症研究中心
Survivin is unique for its expression in human malignancies but not in normal adult cells. It has been implicated in sensitisation to chemotherapy and as a prognostic marker in several common cancers. Immunohistochemistry for Survivin, P53 and BCL-2 expression as well as cell proliferative index (Ki-67) and apoptosis index (TUNEL) was conducted on 52 pancreatic and 12 ampullary adenocarcinomas. Survivin was detected in the cytoplasm of carcinoma cells in 46 (88%) of pancreatic tumours. P53 and BCL-2 were detected in 54% and 12% of pancreatic tumours, respectively. Proliferative index was 26.2±10.5% and apoptosis index was 1.38±0.69%. Prevalence of Survivin expression was significantly higher in P53-positive than in P53-negative cases (P=0.05) but was not associated with BCL-2 expression. Incrementally higher weighted scores of Survivin expression were associated with increased proliferative index (P=0.001). Furthermore, there was linear correlation between increased proliferative index and higher apoptosis index (P<0.001). Surprisingly, higher scores of Survivin expression were associated with increased apoptosis index (P=0.007). Survival characteristics were not influenced by Survivin, P53 or BCL-2 expression, apoptosis index or proliferative index. Ampullary carcinoma showed Survivin expression in 83% of cases. However, unlike pancreatic carcinoma, there was no correlation between Survivin and P53 expression or proliferative index. In conclusion, Survivin is expressed in the majority of pancreatic adenocarcinomas and correlates with both cellular proliferation and apoptosis. Molecular manipulation of Survivin expression may enhance chemotherapy and radiation therapy for pancreatic cancer. British Journal of Cancer (2002) 86, 886–892. DOI: 10.1038/sj/bjc/6600133 www.bjcancer.com © 2002 Cancer Research UK