Protein‐imprinted particles for coronavirus capture from solution
Protein‐imprinted particles for coronavirus capture from solution
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DOI：
10.1002/jssc.202200543
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发表时间：
2022-09
影响因子：
3.1
通讯作者：
Naomi L Senehi;Matthew Ykema;Ruonan Sun;R. Verduzco;L. Stadler;Y. Tao;Pedro J. J. Alvarez-Pedro-J.-J.-Alvarez-2057275828
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文献类型：
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作者：
Naomi L Senehi;Matthew Ykema;Ruonan Sun;R. Verduzco;L. Stadler;Y. Tao;Pedro J. J. Alvarez-Pedro-J.-J.-Alvarez-2057275828
分子印迹是一种选择性吸附病毒的有效方法，但需要识别和验证作为有效印迹模板的表位。在这项工作中，合成了用于冠状病毒捕获的糖蛋白印迹颗粒。吸附在pH 6时达到最大（糖蛋白等电点），其中糖蛋白印迹颗粒优于非印迹颗粒，分别吸附4.96 × 106 ± 3.33 × 103和3.54 × 106 ± 1.39 × 106中位组织培养感染剂量/mg靶冠状病毒，人冠状病毒-器官培养43，在前30 min内（p = 0.012）。在竞争性吸附过程中，通过pH调节（pH 6），糖蛋白印迹颗粒吸附的靶病毒比非靶冠状病毒（人冠状病毒-H163）更多，90 min内去除率为2.34对1.94 log（p < 0.01）。相比之下，非印迹颗粒在靶病毒与非靶病毒清除方面没有显着差异。静电势计算显示，人冠状病毒-器官培养物43糖蛋白在pH 6下具有带正电荷的口袋，这可能有助于在较低pH值下的吸附。因此，通过pH调节调节靶病毒糖蛋白电荷通过最小化与颗粒的排斥性静电相互作用来增强吸附。总体而言，这些结果强调了糖蛋白印记颗粒在捕获冠状病毒方面的有效使用，并辨别了糖蛋白印记在捕获有包膜病毒方面的优点和局限性。
Molecular imprinting is a promising strategy to selectively adsorb viruses, but it requires discerning and validating epitopes that serve as effective imprinting templates. In this work, glycoprotein‐imprinted particles were synthesized for coronavirus capture. Adsorption was maximized at pH 6 (the glycoprotein isoelectric point) where the glycoprotein‐imprinted particles outperformed non‐imprinted particles, adsorbing 4.96 × 106 ± 3.33 × 103 versus 3.54 × 106 ± 1.39 × 106 median tissue culture infectious dose/mg of the target coronavirus, human coronavirus – organ culture 43, within the first 30 min (p = 0.012). During competitive adsorption, with pH adjustment (pH 6), the glycoprotein‐imprinted particles adsorbed more target virus than non‐target coronavirus (human coronavirus – Netherland 63) with 2.34 versus 1.94 log removal in 90 min (p < 0.01). In contrast, the non‐imprinted particles showed no significant difference in target versus non‐target virus removal. Electrostatic potential calculation shows that the human coronavirus – organ culture 43 glycoprotein has positively charged pockets at pH 6, which may facilitate adsorption at lower pH values. Therefore, tuning the target virus glycoprotein charge via pH adjustment enhanced adsorption by minimizing repulsive electrostatic interactions with the particles. Overall, these results highlight the effective use of glycoprotein‐imprinted particles for coronavirus capture and discern the merits and limitations of glycoprotein imprinting for the capture of enveloped viruses.