Molecular determinants during dental morphogenesis and cytodifferentiation: a review.
Molecular determinants during dental morphogenesis and cytodifferentiation: a review.
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发表时间：
1991-10
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通讯作者：
H. Slavkin
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作者：
H. Slavkin
颅面发育为研究形态发生、细胞分化、组织特异性细胞外基质（ECM）形成和生物矿化的细胞和分子生物学提供了许多机会。与下颌骨形态发生，特别是牙齿形成相关的监管过程正在调查的分子决定因素，如细胞粘附分子（CAM），底物粘附分子（SAM），和组织特异性的结构基因产物在连续的发育阶段中表达的识别。基于在无血清、化学成分确定的培养基中进行的体外器官型培养研究，已发现下颌骨和牙齿形态发生和细胞分化都需要指导性和许可性信号。例如，内在的发育指令（自分泌和旁分泌因子），独立于长距离激素或外源性生长因子，介导从牙板起始到牙冠和牙齿发育的初始牙根阶段的形态发生。本文综述了最近的结果，利用实验胚胎学，器官培养，重组DNA技术和免疫细胞学，以阐明机制，指导上皮间充质相互作用与下颌骨形态发生，牙齿的位置信息，随后的牙冠和最初的牙根发育。
Craniofacial development provides a number of opportunities to investigate the cellular and molecular biology of morphogenesis, cytodifferentiation, tissue-specific extracellular matrix (ECM) formations, and biomineralization. Regulatory processes associated with mandibular morphogenesis and specifically tooth formation are being investigated by the identification of when and where molecular determinants such as cell adhesion molecules (CAMs), substrate adhesion molecules (SAMs), and tissue-specific structural gene products are expressed during sequential developmental stages. Based upon in vitro organotypic culture studies in serumless, chemically defined medium, instructive and permissive signaling has been found to be required for both mandibular and dental morphogenesis and cytodifferentiation. For example, intrinsic developmental instructions (autocrine and paracrine factors), independent of long-range hormonal or exogenous growth factors, mediate morphogenesis from the initiation of the dental lamina through crown and initial root stages of tooth development. This review summarizes recent results using experimental embryology, organ culture, recombinant DNA technology, and immunocytology to elucidate mechanisms responsive to instructive epithelial-mesenchymal interactions associated with mandibular morphogenesis, tooth positional information, and subsequent tooth crown and initial root development.