The effects of 50 Hz magnetic field-exposed cell culture medium on cellular functions in FL cells
The effects of 50 Hz magnetic field-exposed cell culture medium on cellular functions in FL cells
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50 Hz 磁场暴露的细胞培养基对 FL 细胞功能的影响
DOI：
10.1093/jrr/rrz020
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发表时间：
2019-07-01
影响因子：
2
通讯作者：
Chen, Guangdi
中科院分区：
文献类型：
--
作者：
Fei, Yue;Su, Liling;Chen, Guangdi
尽管极低频磁场（ELF-MFs）已被国际癌症研究机构（IARC）列为人类可能的致癌物，但其生物学效应和潜在机制仍不清楚。我们以前的研究表明，ELF-MF暴露影响的相对介电常数的盐溶液，这表明MF暴露改变的物理性质的解决方案。为探讨极低频磁场（ELF-MF）生物效应的生物物理机制，采用相位解调表面等离子体共振（SPR）传感技术，研究了0 ~ 4.0 mT的50 Hz正弦磁场对培养液介电常数的影响。然后，使用细胞计数试剂盒-8、蛋白质印迹、流式细胞术、γ H2 AX焦点形成和彗星试验分析MF预暴露培养基对细胞活力、促分裂原活化蛋白激酶（MAPK）信号通路、氧化应激和遗传稳定性的生物学效应。结果表明，MF暴露后SPR信号呈时间和剂量依赖性降低，且降低的SPR信号在MF暴露结束后是可逆的。然而，MF预暴露的培养基没有显着改变细胞活力，细胞内活性氧水平，MARK信号通路的激活，或在人羊膜上皮细胞（FL细胞）的遗传稳定性。总之，我们的数据表明，培养基的相对介电常数的影响，由50 Hz的MF暴露，但这种变化并不影响FL细胞的生物过程。
Although extremely low frequency magnetic fields (ELF-MFs) have been classified as a possible carcinogen for humans by the International Agency for Research on Cancer (IARC), their biological effects and underlying mechanisms are still unclear. Our previous study indicated that ELF-MF exposure influenced the relative permittivity of the saline solution, suggesting that the MF exposure altered physical properties of the solution. To explore the biophysical mechanism of ELF-MF-induced biological effects, this study examined the effects of 50 Hz sinusoidal MF at 0-4.0 mT on the permittivity of culture medium with phase-interrogation surface plasmon resonance (SPR) sensing. Then, the biological effects of MF pre-exposed culture medium on cell viability, the mitogen-activated protein kinase (MAPK) signaling pathways, oxidative stress, and genetic stabilities were analyzed using Cell Counting Kit-8, western blot, flow cytometry, gamma H2AX foci formation, and comet assay. The results showed that SPR signals were decreased under MF exposure in a time- and dose-dependent manner, and the decreased SPR signals were reversible when the exposure was drawn off. However, MF pre-exposed culture medium did not significantly change cell viability, intracellular reactive oxygen species level, activation of the MARK signaling pathways, or genetic stabilities in human amniotic epithelial cells (FL cells). In conclusion, our data suggest that the relative permittivity of culture medium was influenced by 50 Hz MF exposure, but this change did not affect the biological processes in FL cells.