Sex-specific influences of Frankliniella occidentalis (Western Flower Thrips) in the transmission of Tomato Spotted Wilt Virus (Tospovirus)
Sex-specific influences of Frankliniella occidentalis (Western Flower Thrips) in the transmission of Tomato Spotted Wilt Virus (Tospovirus)
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DOI：
10.1007/bf03356562
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发表时间：
2015-12-01
影响因子：
2
通讯作者：
Poehling, Hans-Michael
中科院分区：
文献类型：
--
作者：
Ogada, Pamella Akoth;Poehling, Hans-Michael
番茄斑萎病毒-植物-媒介相互作用是一种特殊而复杂的关系。已经确定，有几个因素影响这种相互作用，导致病毒在病媒群体中传播效率的高度变异性。我们研究了载体性别的贡献，这种相互作用，使用Frankliniella ococcuentalis（西方花蓟马）饲养的番茄斑萎病毒（TSWV）感染或未感染辣椒年叶片在整个幼虫阶段。随后将蛹单独转移到健康叶盘上，以检查所有处理中雄性和雌性之间的存活率、发育率、传播效率和摄食行为的差异。这是基于以下假设：病媒的性别影响病媒种群内的传播效率。我们的研究结果表明，男性有显着更高的寿命，较低的死亡率和较短的发展时间相比，女性，而在比较的摄食行为，女性被发现比男性更密集的饲料，无论他们是否暴露于TSWV。相反，传播效率，这是指成功接种和建立的病毒在宿主植物后，一段时间的接种喂养，被认为是显着较高的男性相比，女性，尽管女性具有最高的百分比传输个人。这些发现部分解释了F. Occidentalis在种群内的传播，进一步了解可能影响F. [咒语]
The tospovirus-plant-vector interaction is a specific and complex relationship. It is established that several factors influence this interaction leading to high variability of transmission efficiency of the virus within a vector population. We studied the contribution of vector gender to this interaction using Frankliniella occidentalis (western flower thrips) reared on either Tomato spotted wilt virus (TSWV) infected or uninfected Capsicum annum leaflets throughout their larval stages. Later pupae were individually transferred onto healthy leaf disks to examine the differences in: survival, development rate, transmission efficiency and feeding behavior between males and females in all the treatments. This was based on the hypothesis that sex of the vector influences transmission efficiency within the vector population. Our results showed that males had significantly higher longevity, lower mortality and shorter development time compared to females, while in the comparison of feeding behaviour, females were found to feed more intensively than males, regardless of whether they were exposed or unexposed to TSWV. Conversely, transmission efficiency; which referred to successful inoculation and establishment of the virus in the host plant after a given period of inoculative feeding, was found to be significantly higher in males compared to females, despite females having the highest percentage of transmitting individuals. These findings account in part for the high variability in vector competence of F. occidentalis within a population, which further give insight into the biological factors that may influence the transmission of TSWV by F. occidentalis.