The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis
The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis
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通过变性梯度凝胶电泳和 16S rDNA 测序分析揭示沼气池中细菌和古菌群落的结构
DOI：
10.1111/j.1365-2672.2008.04064.x
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Zhou, Z. H.
中科院分区：
文献类型：
--
作者：
Liu, F. H.;Wang, S. B.;Zhou, Z. H.
为鉴定中温沼气池处理猪粪过程中的细菌和古菌组成，比较两种16 SrDNA方法对微生物结构研究的一致性，通过对两个16 SrDNA克隆文库进行测序，鉴定出69个细菌操作分类单位（OTU）和25个古菌OTU。多数OTU被鉴定为厚壁菌门（占总克隆的47.2%）、拟杆菌门（35.4%）和螺旋体门（13.2%）。优势产甲烷菌为伯金甲烷球菌（29.0%）、巴氏甲烷八叠球菌（27.4%）和亨氏甲烷菌（10.8%）。只有9%的细菌和20%的古细菌OTU与培养的分离株匹配，相似性指数>= 97%。约78%的优势细菌16 SrDNAV 3区变性梯度凝胶电泳（DGGE）结果表明，在消化池中，绝大多数细菌和古细菌都是未培养的，厚壁菌门（Firmicutes）、拟杆菌门（Bacteroides）和螺旋体门（Spirochaeus）的细菌似乎负责纤维素分解、蛋白质分解、产酸、还原硫和同型乙酸;结果表明，DGGE图谱反映了农村沼气池中微生物的总体结构，DGGE图谱能够反映沼气池中微生物的优势菌群。
To identify the bacterial and archaeal composition in a mesophilic biogas digester treating pig manure and to compare the consistency of two 16S rDNA-based methods to investigate the microbial structure.Sixty-nine bacterial operational taxonomic units (OTU) and 25 archaeal OTU were identified by sequencing two 16S rDNA clone libraries. Most bacterial OTU were identified as phyla of Firmicutes (47.2% of total clones), Bacteroides (35.4%) and Spirochaetes (13.2%). Methanoculleus bourgensis (29.0%), Methanosarcina barkeri (27.4%) and Methanospirillum hungatei (10.8%) were the dominant methanogens. Only 9% of bacterial and 20% of archaeal OTU matched cultured isolates at a similarity index of >= 97%. About 78% of the dominant bacterial (with abundance > 3%) and 83% of archaeal OTU were recovered from the denaturing gradient gel electrophoresis (DGGE) bands of V3 regions in 16S rDNAs.In the digester, most bacterial and archaeal species were uncultured; bacteria belonging to Firmicutes, Bacteroides and Spirochaetes seem to take charge of cellulolysis, proteolysis, acidogenesis, sulfur-reducing and homoacetogenesis; the most methanogens were typical hydrogenotrophic or hydrogenotrophic/aceticlastic; DGGE profiles reflected the dominant microbiota.This study gave a first insight of the overall microbial structure in a rural biogas digester and also indicated DGGE was useful in displaying its dominant microbiota.