Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95.
Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95.
复制标题
柯萨奇病毒和腺病毒受体 (CAR) 通过 PSD-95 介导酸感应离子通道 3 (ASIC3) 的运输。
DOI：
10.1016/j.bbrc.2012.07.033
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发表时间：
2012
影响因子：
3.1
通讯作者：
Benson,ChristopherJ
中科院分区：
文献类型：
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作者：
Excoffon,KatherineJDA;Kolawole,AbimbolaO;Kusama,Nobuyoshi;Gansemer,NicholasD;Sharma,Priyanka;Hruska-Hageman,AlesiaM;Petroff,Elena;Benson,ChristopherJ
我们之前已经证明柯萨奇病毒和腺病毒受体（CAR）可以与突触后密度95（PSD-95）相互作用并将PSD-95定位到细胞与细胞的连接处。我们还表明，酸传感离子通道 (ASIC3) 是一种在机械感觉和疼痛信号传导中发挥作用的 H+ 门控阳离子通道，其活性受到 PSD-95 通过基于 PDZ 的相互作用的负调节。我们询问 CAR 和 ASIC3 是否同时与 PSD-95 相互作用，如果是，这些蛋白质的共表达是否会改变它们的细胞分布和定位。结果表明，CAR 和 ASIC3 仅在与 PSD-95 共表达时才会发生共免疫沉淀。 CAR 还将 PSD-95 和 ASIC3 带到异源细胞的连接处。此外，CAR 还可以挽救 PSD-95 介导的 ASIC3 电流抑制。这些数据表明，除了作为病毒受体和粘附分子的活性外，CAR 还可以在基于 PDZ 的支架复合物中运输蛋白质（包括离子通道）方面发挥作用。
We have previously shown that the Coxsackievirus and adenovirus receptor (CAR) can interact with post-synaptic density 95 (PSD-95) and localize PSD-95 to cell–cell junctions. We have also shown that activity of the acid sensing ion channel (ASIC3), a H+-gated cation channel that plays a role in mechanosensation and pain signaling, is negatively modulated by PSD-95 through a PDZ-based interaction. We asked whether CAR and ASIC3 simultaneously interact with PSD-95, and if so, whether co-expression of these proteins alters their cellular distribution and localization. Results indicate that CAR and ASIC3 co-immunoprecipitate only when co-expressed with PSD-95. CAR also brings both PSD-95 and ASIC3 to the junctions of heterologous cells. Moreover, CAR rescues PSD-95-mediated inhibition of ASIC3 currents. These data suggest that, in addition to activity as a viral receptor and adhesion molecule, CAR can play a role in trafficking proteins, including ion channels, in a PDZ-based scaffolding complex.