The nucleotide sequence of koala (Phascolarctos cinereus) retrovirus:: a novel type C endogenous virus related to gibbon ape leukemia virus
The nucleotide sequence of koala (Phascolarctos cinereus) retrovirus:: a novel type C endogenous virus related to gibbon ape leukemia virus
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DOI：
10.1128/jvi.74.9.4264-4272.2000
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Robinson, WF
中科院分区：
文献类型：
--
作者：
Hanger, JJ;Bromham, LD;Robinson, WF
一种新的逆转录病毒，形态上一致，与哺乳动物C型逆转录病毒，通过电子显微镜检测有丝分裂原刺激的外周血单个核细胞培养物从163考拉和淋巴瘤组织从3考拉。从17只野生和圈养考拉的血液和组织中PCR扩增出前病毒，逆转录酶-PCR证实了考拉血清和细胞培养上清液中的病毒mRNA、病毒基因组RNA和逆转录酶活性。来自基因组DNA和mRNA的病毒序列的比较表明同一性指示单一的逆转录病毒物种-这里称为考拉逆转录病毒（KoRV），用标记的KoRV探针对考拉组织基因组DNA进行Southern印迹分析，结果显示与内源性逆转录病毒一致的条带。在临床正常的考拉和造血系统疾病的考拉的DNA中检测到完整的和明显截短的前病毒。在其他有袋类动物中未检测到KoRV相关病毒，系统发育分析显示KoRV与巨猿白血病病毒（GALV）矛盾地簇集。GALV和KoRV之间的高度相似性表明，这些病毒密切相关，最近发生了跨宿主传播。报道了KoRV前病毒DNA全序列。
A novel retrovirus, morphologically consistent,vith mammalian C-type retroviruses, was detected by electron microscopy in mitogen-stimulated peripheral blood mononuclear cell cultures from 163 koalas and in lymphoma tissue from 3 koalas. PCR amplified provirus from the blood and tissues of 17 wild and captive koalas, and reverse transcriptase-PCR demonstrated viral mRNA, viral genomic RNA, and reverse transcriptase activity in koala serum and cell culture supernatants, Comparison of viral sequences derived from genomic DNA and mRNA showed identity indicative of a single retroviral species-here designated koala retrovirus (KoRV), Southern blot analysis of koala tissue genomic DNA using labelled KoRV probes demonstrated banding consistent with an endogenous retrovirus. Complete and apparently truncated proviruses were detected in DNA of both clinically normal koalas and those with hematopoietic disease. KoRV-related viruses were not detected in other marsupials, and phylogenetic analysis showed that KoRV paradoxically clusters with gibbon ape leukemia virus (GALV). The strong similarity between GALV and KoRV suggests that these viruses are closely related and that recent cross-host transmission has occurred. The complete proviral DNA sequence of KoRV is reported.