Accumulation of S Phase Populations of FM3A Cells Growing in the Mouse due to Administration of 5-Fluoro-2'-Deoxyuridine
Accumulation of S Phase Populations of FM3A Cells Growing in the Mouse due to Administration of 5-Fluoro-2'-Deoxyuridine
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由于施用 5-氟-2-脱氧尿苷，小鼠中生长的 FM3A 细胞 S 期群体的积累
DOI：
10.1247/csf.6.357
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
M. Yamada
中科院分区：
文献类型：
--
作者：
F. Hanaoka;K. Nagata;Yoshinari Watanabe;T. Enomoto;M. Yamada
我们已经建立了在小鼠中生长的FM 3A细胞的S期群体的积累程序。腹腔内接种1 × 106个FM 3A细胞后，将小鼠饲养5天，然后用100 μg 5-氟-2 '-脱氧尿苷处理16小时。当从小鼠中取出FM 3A细胞并在体外培养时，超过70%的细胞合成DNA，并且在14小时后细胞数量增加了1.8倍。DEAE-纤维素柱层析的核提取物馏分表明，同步化的细胞含有约3倍的活性的DNA聚合酶比随机细胞。细胞质部分中的活性保持在相同水平。
We have established procedures for the accumulation of an S phase population of FM3A cells grown in the mouse. Mice were kept for 5 days after intraperitoneal inoculation with 1 x 106 FM3A cells, then they were treated with 100 μg of 5-fluoro-2'-deoxyuridine for 16 h. When the FM3A cells were taken from the mice and cultured in vitro, more than 70% of the cells synthesized DNA, and the cell number increased 1.8-fold after 14 h. DEAE-cellulose column chromatography of the nuclear extract fraction showed that the synchronized cells contained about three times the activity for DNA polymerase a than that of random cells. Activity in the cytoplasmic fraction remained at the same level.