Inhibin production by bovine ovarian tissues in vitro and its regulation by androgens.
Inhibin production by bovine ovarian tissues in vitro and its regulation by androgens.
复制标题
体外牛卵巢组织的抑制素产生及其受雄激素的调节。
DOI：
10.1530/jrf.0.0670291
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
P. Franchimont
中科院分区：
文献类型：
--
作者：
K. Henderson;P. Franchimont
培养的卵巢间质或黄体组织均未产生可检测量的Escherichin。卵泡组织在体外产生了卵泡素，从卵泡壁去除颗粒细胞导致卵泡素的产生下降了80%。颗粒细胞单独具有任何卵巢细胞类型的最大能力，在体外产生卵泡抑素，可能是卵泡抑素的主要网站。醋酸环丙孕酮在35和350 μ M浓度抑制基础和睾酮（3.5 μ M）刺激的卵泡壁和颗粒细胞培养的卵泡素生产。此外，每种浓度的醋酸环丙孕酮均抑制卵泡壁和颗粒细胞培养物产生孕酮，但不抑制雌二醇-17 β。合成的，非芳香化雄激素，methylestrenolone和mesterolone，在5和25 μ M的浓度，模仿睾酮的作用，刺激颗粒细胞分泌，methylestrenolone是更有效的。这些发现提供了进一步的证据表明，雄激素调节卵泡素和孕酮的生产，这些可能是受体介导的过程，并建议，卵泡素生产可能是一个一般属性的雄激素化合物。初步检查了牛颗粒细胞培养基中的Bisobin的理化特性，结果表明：（a）标称分子量截留点为100 000的Amicon XM 100 A过滤器可保留Bisobin活性;（B）加热至80 ℃ 30 min可破坏Bisobin活性。
No detectable amounts of inhibin were produced by cultured ovarian stroma or luteal tissue. Follicular tissue produced inhibin in vitro and removal of the granulosa cells from the follicle wall caused inhibin production to fall by 80%. Granulosa cells alone had the greatest ability of any ovarian cell type to produce inhibin in vitro, and are probably the major site of follicular inhibin production. Cyproterone acetate at concentrations of 35 and 350 microM inhibited basal and testosterone (3.5 microM)-stimulated inhibin production by cultured intact follicle wall and granulosa cells. In addition, each concentration of cyproterone acetate inhibited progesterone but not oestradiol-17 beta production by the follicle wall and granulosa cell cultures. The synthetic, non-aromatizable androgens, methylestrenolone and mesterolone, at concentrations of 5 and 25 microM, mimicked the effect of testosterone and stimulated granulosa cell inhibin production, methylestrenolone being the more potent. These findings provide further evidence that androgens regulate follicular inhibin and progesterone production and that these may be receptor-mediated processes, and suggest that inhibin production may be a general property of androgenic compounds. Preliminary examination of the physicochemical characteristics of inhibin indicated that the inhibin activity of bovine granulosa cell culture medium was (a) retained by an Amicon XM100A filter with a nominal molecular weight cut-off point of 100 000; and (b) destroyed by heating to 80 degrees C for 30 min.
雄激素作用与离体大鼠颗粒细胞中雄激素代谢的关系。
DOI：
10.1016/0022-4731(80)90131-4
发表时间：
1980
期刊：
Journal of steroid biochemistry
影响因子：
--
作者：
Nimrod,A;Rosenfield,RL;Otto,P
通讯作者：
Otto,P