A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System.
A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System.
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DOI：
10.1038/srep39202
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发表时间：
2017-01-04
影响因子：
4.6
通讯作者：
Wang JY
中科院分区：
文献类型：
--
作者：
Geng S;Wang Y;Wang L;Kouyama T;Gotoh T;Wada S;Wang JY
采用冷冻透射电镜和同步辐射小角X射线研究了阳离子偶氮苯衍生物、4-胆固醇羰基-4′-(N,N,N-三乙胺丁氧基溴)偶氮苯(CAB)和表面活性剂十二烷基硫酸钠(SDS)在水溶液中形成的自组装体的结构。 散射（SAXS）。可以观察到单层和多层囊泡。囊泡中的 CAB 在紫外线或可见光照射下能够发生可逆的反式异构化为顺式异构化。 SAXS可以捕捉到紫外光照射下的结构变化，这表明顺式多层囊泡的层间距增加了0.2-0.3nm。基于这种微观结构的变化，紫外线照射可以触发罗丹明 B (RhB) 和阿霉素 (DOX) 的释放。当将 NIH 3T3 细胞和 Bel 7402 细胞与负载 DOX 的 CAB/SDS 囊泡一起孵育时，与未照射的细胞相比，紫外线照射诱导的 DOX 释放显着降低了两种细胞系的活力。体外实验表明CAB/SDS囊泡能够高效地将负载分子递送至细胞内。体内实验表明，CAB/SDS囊泡不仅对大鼠视网膜具有较高的药物递送效率，而且可以较长时间维持较高的药物浓度。 CAB/SDS 阴阳离子囊泡可能会作为光控释放的智能药物递送系统找到潜在的应用。
The structure of a self-assembly formed from a cationic azobenzene derivative, 4-cholesterocarbonyl-4′-(N,N,N-triethylamine butyloxyl bromide) azobenzene (CAB) and surfactant sodium dodecyl sulfate (SDS) in aqueous solution was studied by cryo-TEM and synchrotron radiation small-angle X-ray scattering (SAXS). Both unilamellar and multilamellar vesicles could be observed. CAB in vesicles were capable to undergo reversible trans-to-cis isomerization upon UV or visible light irradiation. The structural change upon UV light irradiation could be catched by SAXS, which demonstrated that the interlamellar spacing of the cis-multilamellar vesicles increased by 0.2–0.3 nm. Based on this microstructural change, the release of rhodamine B (RhB) and doxorubicin (DOX) could be triggered by UV irradiation. When incubated NIH 3T3 cells and Bel 7402 cells with DOX-loaded CAB/SDS vesicles, UV irradiation induced DOX release decreased the viability of both cell lines significantly compared with the non-irradiated cells. The in vitro experiment indicated that CAB/SDS vesicles had high efficiency to deliver loaded molecules into cells. The in vivo experiment showed that CAB/SDS vesicles not only have high drug delivery efficiency into rat retinas, but also could maintain high drug concentration for a longer time. CAB/SDS catanionic vesicles may find potential applications as a smart drug delivery system for controlled release by light.
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