Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells
Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells
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DOI：
10.1634/stemcells.22-3-313
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发表时间：
2004-01-01
期刊：
影响因子：
5.2
通讯作者：
Cheung, HS
中科院分区：
文献类型：
--
作者：
Huang, CYC;Hagar, KL;Cheung, HS
本研究的目的是研究周期性压力负荷对兔骨髓间充质干细胞（BM-MSCs）在琼脂糖培养中向软骨细胞分化的影响。从新西兰白色兔的胫骨和股骨获得兔BM-MSC。在通过沉淀培养物验证BM-MSC的软骨形成潜力后，通过将BM-MSC悬浮在2%琼脂糖（10（7）个细胞/ml）中制备细胞-琼脂糖构建体，用于在定制的生物反应器中进行的循环无限制压缩测试。标本分为四组：对照组;转化生长因子（TGF-β）（TGF-β 1处理）;负载（刺激循环，无侧限压缩载荷）;和TGF-β负载（TGF-β 1处理和负载刺激）组。在加载实验中，样本经受正弦加载，10%的应变幅度，频率为1 Hz，每天4小时。实验连续进行3、7和14天。虽然实验组（TGF-β、负载和TGF-β负载）在三个时间段表现出显著更高水平的软骨形成标志物（胶原II和聚集蛋白聚糖）表达，但在额外的5天培养后，实验组之间没有差异。这表明单独的压缩负荷与TGF-β或TGF-β加负荷处理一样有效地诱导兔BM-MSC的软骨形成分化。此外，无论是压缩负荷和TGF-β 1处理被发现促进兔BM-MSCs的TGF-β 1基因表达。结论：周期性压力负荷可通过诱导TGF-β 1的合成促进兔BM-MSCs向软骨细胞分化，从而促进BM-MSCs向软骨细胞分化。
The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2% agarose (10(7) cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-beta) (with TGF-beta1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-beta loading (with TGF-beta1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10% strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-beta, loading, and TGF-beta loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-beta or TGF-beta plus loading treatment. Moreover, both the compressive loading and the TGF-beta1 treatment were found to promote the TGF-beta1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BMMSCs by inducing the synthesis of TGF-beta1, which can stimulate the BM-MSCs to differentiate into chondrocytes.