OCCURRENCE OF GLYCOLATE DEHYDROGENASE AND GLYCOLATE OXIDASE IN GREEN PLANTS - EVOLUTIONARY SURVEY
OCCURRENCE OF GLYCOLATE DEHYDROGENASE AND GLYCOLATE OXIDASE IN GREEN PLANTS - EVOLUTIONARY SURVEY
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DOI：
10.1104/pp.52.4.318
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发表时间：
1973-01-01
期刊：
影响因子：
7.4
通讯作者：
TOLBERT, NE
中科院分区：
文献类型：
--
作者：
FREDERICK, SE;GRUBER, PJ;TOLBERT, NE
分析了各种低地植物、水生被子植物和绿色藻类的匀浆中乙醇酸氧化酶（一种存在于高等植物绿色叶中的过氧化物酶）和乙醇酸脱氢酶（一种某些绿色藻类的功能类似酶）。所有被检测的低地植物的绿色组织（包括苔藓、地钱、蕨类植物和蕨类植物），以及三种淡水水生被子植物，都含有一种类似乙醇酸氧化酶的酶，因为它除了氧化乙醇酸外，还氧化l-乳酸，但不氧化d-乳酸，并且对2 mm氰化物不敏感。许多绿色藻类（包括先前声称具有乙醇酸氧化酶的普通小球藻）含有一种类似于乙醇酸脱氢酶的酶，因为它氧化d-而不是l-乳酸，并被2 mm氰化物抑制。其他绿色藻类具有乙醇酸氧化酶的活性特征，因此，表现出大量的乙醇酸依赖的O2摄取。乙醇酸氧化酶和乙醇酸脱氢酶在绿色植物中的这种分布模式可能具有系统发育的意义。过氧化氢酶（过氧化物酶体的标志酶）的活性在藻类中普遍低于陆生植物和水生被子植物。然而，在藻类中，过氧化氢酶的水平和氧化乙醇酸的酶的类型之间没有一致的相关性。
Homogenates of various lower land plants, aquatic angiosperms, and green algae were assayed for glycolate oxidase, a peroxisomal enzyme present in green leaves of higher plants, and for glycolate dehydrogenase, a functionally analogous enzyme characteristic of certain green algae. Green tissues of all lower land plants examined (including mosses, liverworts, ferns, and fern allies), as well as three freshwater aquatic angiosperms, contained an enzyme resembling glycolate oxidase, in that it oxidizedl- but notd-lactate in addition to glycolate, and was insensitive to 2 mmcyanide. Many of the green algae (includingChlorella vulgaris, previously claimed to have glycolate oxidase) contained an enzyme resembling glycolate dehydrogenase, in that it oxidizedd- but notl-lactate, and was inhibited by 2 mmcyanide. Other green algae had activity characteristic of glycolate oxidase and, accordingly, showed a substantial glycolate-dependent O2uptake. It is pointed out that this distribution pattern of glycolate oxidase and glycolate dehydrogenase among the green plants may have phylogenetic significance.Activities of catalase, a marker enzyme for peroxisomes, were also determined and were generally lower in the algae than in the land plants or aquatic angiosperms. Among the algae, however, there were no consistent correlations between levels of catalase and the type of enzyme which oxidized glycolate.