METASTATIC TUMOR-CELLS ADHERE PREFERENTIALLY TO THE EXTRACELLULAR-MATRIX UNDERLYING VASCULAR ENDOTHELIAL-CELLS
METASTATIC TUMOR-CELLS ADHERE PREFERENTIALLY TO THE EXTRACELLULAR-MATRIX UNDERLYING VASCULAR ENDOTHELIAL-CELLS
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DOI：
10.1002/ijc.2910260516
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发表时间：
1980-01-01
影响因子：
6.4
通讯作者：
NICOLSON, GL
中科院分区：
文献类型：
--
作者：
KRAMER, RH;GONZALEZ, R;NICOLSON, GL
研究了两种转移性细胞系，小鼠B16 - FI黑色素瘤细胞和人Hs939黑色素瘤细胞，检测它们黏附于融合的血管内皮细胞单层以及其下的内皮细胞外基质的能力。肿瘤细胞缓慢附着于内皮细胞单层，而它们能迅速黏附于分离的细胞外基质。当用十二烷基硫酸钠溶液中的聚丙烯酰胺凝胶电泳分析时，细胞外基质主要由一种在迁移和分子量上与纤连蛋白相同的蛋白质组成。肿瘤细胞在纤连蛋白包被的聚乙烯表面的黏附模拟了肿瘤细胞在细胞外基质上的快速附着速率，并且黏附于细胞外基质或纤连蛋白包被的聚乙烯培养皿的肿瘤细胞呈现出一种不寻常的、高度铺展且扁平的形态，带有许多小的突起。与内皮基底膜相关的纤连蛋白可能在一定程度上负责建立一种黏附梯度，这在恶性细胞渗出过程中可能是重要的。
Two metastatic cell lines, mouse B16-FI melanoma and human Hs939 melanoma, were examined for their abilities to adhere to confluent vascular endothelial cell monolayers and to the underlying endothelial extracellular matrix. Tumor cells attached slowly to the endothelial cell monolayers while they adhered rapidly to isolated extracellular matrix. When analyzed by polyacrylamide gel electrophoresis in sodium dodecylsulfate soltions, the extracellular matrix was primarily composed of a protein of identical migration and MW to fibronectin. Tumor-cell adhesion to fibronectin-coated polyvinyl surfaces mimicked the rapid rate of attachment of tumor cells to extracellular matrix and tumor cells adherent to extracellular matrix or fibronectin-coated polyvinyl dishes adopted an unusual, highly spread and flattened morphology with numerous small projections. Fibronectin associated with the endothelial basement membrane may be, in part, responsible for establishing an adhesive gradient that could be important in malignant cell extravasation.