Genetic Manipulation of Neisseria gonorrhoeae.
Genetic Manipulation of Neisseria gonorrhoeae.
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DOI：
10.1002/9780471729259.mc04a02s23
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发表时间：
2011-11
影响因子：
--
通讯作者：
Dillard JP
中科院分区：
文献类型：
--
作者：
Dillard JP
性传播的病原体淋病奈瑟氏菌会高频率地进行自然转化。这一特性导致了抗生素耐药性标记的快速传播和淋球菌群体的泛菌结构。然而，高频变换也使得N.淋病是最容易在实验室中进行基因操作的细菌种类之一。已经开发了导致转化频率> 50%的技术，允许通过筛选而非选择来鉴定突变体。构建体已经被创建以利用这种高频率转化，促进遗传突变、互补和异源基因表达。描述了N.淋病，以及这种挑剔的生物体的生长。
The sexually-transmitted pathogen, Neisseria gonorrhoeae, undergoes natural transformation at high frequency. This property has led to the rapid dissemination of antibiotic resistance markers and to the panmictic structure of the gonococcal population. However, high frequency transformation also makes N. gonorrhoeae one of the easiest bacterial species to manipulate genetically in the laboratory. Techniques have been developed that result in transformation frequencies >50%, allowing the identification of mutants by screening and without selection. Constructs have been created to take advantage of this high frequency transformation, facilitating genetic mutation, complementation, and heterologous gene expression. Techniques are described for genetic manipulation of N. gonorrhoeae, as well as for growth of this fastidious organism.