INDUCTION OF EXPERIMENTAL ALLERGIC NEURITIS WITH A PEPTIDE FROM MYELIN-P2 BASIC-PROTEIN
INDUCTION OF EXPERIMENTAL ALLERGIC NEURITIS WITH A PEPTIDE FROM MYELIN-P2 BASIC-PROTEIN
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DOI：
10.1038/268752a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
POWERS, JM
中科院分区：
文献类型：
--
作者：
BROSTOFF, SW;LEVIT, S;POWERS, JM
EXPERIMENTAL allergic neuritis (EAN) is an autoimmune demyelinating disease of the peripheral nervous system (PNS) produced in animals by injection of homogenates of PNS tissue1or PNS myelin2emulsified with Freund's complete adjuvant. Although it has been produced in such species as rabbits1, guinea pigs3, and mice3, the neuritogenic determinant has not been identified. In experimental allergic encephalomyelitis (EAE), the central nervous system (CNS) counterpart of EAN, the encephalitogen is the myelin basic protein4. PNS myelin has two basic proteins, P1and P25, and the latter has been suggested to be the neuritogen2. In attempts to induce the disease with intact P2(refs 6–9), mild symptoms of EAN were occasionally produced, but these were inconsistent. P2was implicated as the neuritogen when circulating lymphocytes of animals with EAN induced by injection of whole PNS myelin10were found to be sensitised to P2but not P1. Physicochemical studies of P2have demonstrated a very stableβstructure in aqueous solution11. Because the conformation is likely to differ in solution and in intact myelin, it is likely that the neuritogenic determinant(s) are altered. We have, therefore, tested peptides derived from the protein in order to minimise alteration of any neuritogenic determinant by extraneous port s of the intact molecule. We have identified a neuritogenic determinant within P2—within a peptide representing the NH2-terminal 21 amino acids of P2.
