Conversion of a Fe2S2 Ferredoxin into a Ga3+ Rubredoxin
Conversion of a Fe2S2 Ferredoxin into a Ga3+ Rubredoxin
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Fe2S2 铁氧还蛋白转化为 Ga3 红氧还蛋白
DOI：
10.1021/ja00129a040
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发表时间：
1995
影响因子：
15
通讯作者：
B. Chait
中科院分区：
文献类型：
--
作者：
S. Kazanis;T. Pochapsky;T. Barnhart;J. Penner‐Hahn;U. Mirza;B. Chait
报道了用Ga(sup3+)对恶臭还蛋白(PDX)进行重组，得到了稳定的类Rubredooxin(GaS{sub4})镓衍生物(GaPdx)，它保留了天然PDX的二级结构和整体折叠结构。GaPdx是用Tsibra等人的重组程序制备的。只是FeCl{sub3}被GaCl{sub3}取代。通过比较重构前后的核磁共振信号强度估计，重构后GaPdx的产率高于90%。参考文献16，图3。
We report that reconstitution of putidaredoxin (Pdx) using Ga{sup 3+} yields a stable mononuclear rubredoxin-like (GaS{sub 4}) gallium derivative of Pdx (GaPdx) which retains the secondary structure and global fold of native Pdx. GaPdx was prepared using the reconstitution procedure of Tsibris et al. except that FeCl{sub 3} was replaced with GaCl{sub 3}. The reconstitution results in better than 90% yield of GaPdx, as estimated by comparison of {sup 1}H NMR signal intensities before and after reconstitution. 16 refs., 3 figs.