ACUTE-PHASE RESPONSE IN EXERCISE .3. NEUTROPHIL AND IL-1-BETA ACCUMULATION IN SKELETAL-MUSCLE
ACUTE-PHASE RESPONSE IN EXERCISE .3. NEUTROPHIL AND IL-1-BETA ACCUMULATION IN SKELETAL-MUSCLE
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DOI：
10.1152/ajpregu.1993.265.1.r166
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发表时间：
1993-07-01
影响因子：
--
通讯作者：
CANNON, JG
中科院分区：
文献类型：
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作者：
FIELDING, RA;MANFREDI, TJ;CANNON, JG
9名未经训练的男性（22-29岁）进行了45分钟的下坡跑（16%的坡度，70%的最大心率）。在运动前、运动后45分钟和运动后5天分别对股外侧肌进行针吸活检。免疫组织化学染色显示，运动后即刻肌肉白细胞介素1 β（IL-1 β）增加了135%，运动后5天增加了250%（P < 0.03）。使用中性粒细胞存在的等级量表（0-3），光学显微镜检查显示，在运动后45分钟和运动后5天采集的肌肉活检中，中性粒细胞显著积聚[运动前：0.5 +/-0.2，运动后45分钟：1.5 +/-0.3（P < 0.01），5天后：1.2 +/- 0.2（P < 0.04）]。此外，电子显微镜分析显示受损Z带相对于总Z带的百分比增加[前：4.8 +/-3.5%，后45分钟：32.5 +/- 8.6%（P < 0.05），后5天：14.1 +/- 3.2%]。神经元聚集与细胞内Z带损伤呈正相关（rho = 0.66，P < 0.001）。IL-1 β的免疫组化染色与肌肉中中性粒细胞聚集（rho = 0.38，P < 0.06）和血浆肌酸激酶水平（rho = 0.416，P < 0.04）相关。这些数据表明，离心运动后骨骼肌超微结构损伤与中性粒细胞浸润和肌肉IL-1 β积累有关。
Nine untrained men (22-29 yr) performed 45 min of downhill running (16% incline, 70% of maximum heart rate). Needle biopsies of the vastus lateralis were performed before, 45 min after, and 5 days after exercise. Immunohistochemical staining of muscle cross sections revealed a 135% increase in muscle interleukin-1beta (IL-1beta) immediately after and a 250% increase (P < 0.03) 5 days after exercise. Using a rating scale (0-3) for the presence of neutrophils, light microscopic examination showed a significant accumulation of neutrophils in muscle biopsies taken 45 min after and 5 days after exercise [before: 0.5 +/- 0.2, 45 min after: 1.5 +/- 0.3 (P < 0.01), and 5 days after: 1.2 +/- 0.2 (P < 0.04)]. In addition, electron microscopic analysis showed an increase in the percentage of damaged Z-bands relative to total Z-bands [before: 4.8 +/- 3.5%, 45 min after: 32.5 +/- 8.6% (P < 0.05), and 5 days after: 14.1 +/- 3.2%]. Neutrophil accumulation was positively correlated to intracellular Z-band damage (rho = 0.66, P < 0.001). Immunohistochemical staining for IL-1beta was related to neutrophil accumulation in muscle (rho = 0.38, P < 0.06) and to plasma creatine kinase levels (rho = 0.416, P < 0.04). These data indicate that after eccentric exercise ultrastructural damage to skeletal muscle is associated with neutrophil infiltration and muscle IL-1beta accumulation.