ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
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DOI：
10.1016/s0092-8674(03)00158-2
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发表时间：
2003-03-21
期刊：
影响因子：
64.5
通讯作者：
Patapoutian, A
中科院分区：
文献类型：
--
作者：
Story, GM;Peier, AM;Patapoutian, A
哺乳动物通过周围神经系统中的特殊神经元来检测温度。四个TRPV类通道被牵连到感热，一个TRPM类通道被牵连到感冷。激活这些通道的综合温度范围覆盖了大多数哺乳动物感受到的大部分相关生理光谱，在有害的寒冷范围内存在显著差距。在这里，我们描述了ANKTM1的特征，这是一种冷激活通道，与冷和薄荷醇受体TRPM8相比，其激活温度更低。ANKTM1是Trp通道的远亲家族成员，与TRPM8的氨基酸相似性很小。它存在于部分伤害性感觉神经元中，与TRPV1/VR1(辣椒素/热感受器)共表达，但不表达TRPM8。与ANKTM1的表达一致，我们发现了有毒的冷敏感感觉神经元，这些神经元也对辣椒素有反应，但对薄荷醇没有反应。
Mammals detect temperature with specialized neurons in the peripheral nervous system. Four TRPV-class channels have been implicated in sensing heat, and one TRPM-class channel in sensing cold. The combined range of temperatures that activate these channels covers a majority of the relevant physiological spectrum sensed by most mammals, with a significant gap in the noxious cold range. Here, we describe the characterization of ANKTM1, a cold-activated channel with a lower activation temperature compared to the cold and menthol receptor, TRPM8. ANKTM1 is a distant family member of TRP channels with very little amino acid similarity to TRPM8. It is found in a subset of nociceptive sensory neurons where it is coexpressed with TRPV1/VR1 (the capsaicin/heat receptor) but not TRPM8. Consistent with the expression of ANKTM1, we identify noxious cold-sensitive sensory neurons that also respond to capsaicin but not to menthol.